000			03592nam a22003615i 4500
001			234011
003			ES-VaUE
005			20221220020535.0
007			cr nn 008mamaa
008			170327s2017 xxu| s |||| 0|eng d
020			_a9781493967162
024	7		_a10.1007/978-1-4939-6716-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPromoter Associated RNA _bMethods and Protocols _cedited by Sara Napoli.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (X, 287 páginas) _b283 ilustraciones, 182 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1543
505	0		_aChIP-seq for the Identification of Functional Elements in the Human Genome -- Identification of Candidate Functional Elements in the Genome from ChIP-seq Data -- GRO-seq, A Tool for Identification of Transcripts Regulating Gene Expression.-NanoCAGE: A Method for the Analysis of Coding and Non-coding 5'-capped Transcriptomes -- Deep Cap Analysis of Gene Expression (CAGE): Genome-Wide Identification of Promoters, Quantification of their Activity Andtranscriptional Network Inference -- Deep-RACE: Comprehensive Search for Novel ncRNAs Associated to a Specific Locus -- In silico Prediction of RNA Secondary Structure -- Computational Prediction of RNA-Protein Interactions -- Isolation of Nuclear RNA-Associated Protein Complexes -- Identification of Long Non-Coding RNAs Associated to Human Disease Susceptibility -- Targeting Promoter-associated RNAs by siRNAs -- RNA-FISH to Study Regulatory RNA at the Site of Transcription -- Detection and Characterization of R Loop Structures -- Induction of Transcriptional Gene Silencing by Expression of shRNA Directed to c-Myc P2 Promoter in Hepatocellular Carcinoma by Tissue Specific Virosomal Delivery -- Targeting Promoter Associated Non-coding RNA In Vivo -- Manipulation of Promoter-Associated Noncoding RNAs in Mouse Early Embryos for Controlling Sequence-Specific Epigenetic Status. .
520			_aThis volume is divided in four sections; covering genome wide approaches, techniques for characterize of paRNA structural features are described, selecting pa-RNA, and paRNA therapeutic potential. Chapters describe how siRNAsdirected against paRNAs can be applied in vivo to modulate transcription of important genes controlled by paRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Promoter Associated RNA: Methods and Protocols aims to demonstrate paRNAs as new class of regulatory molecules, to further investigate and value as tools for fine transcriptional tuning.
700	1		_aNapoli, Sara _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493967148
776	0	8	_iPrinted edition: _z9781493967155
776	0	8	_iPrinted edition: _z9781493982813
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6716-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234011 _d234011