000			06206nam a22003615i 4500
001			234012
003			ES-VaUE
005			20221220020535.0
007			cr nn 008mamaa
008			100301s2003 xxu| s |||| 0|eng d
020			_a9781592593583
024	7		_a10.1385/1592593585 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDrugs of Abuse _bNeurological Reviews and Protocols _cedited by John Q. Wang.
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (XIV, 514 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v79
505	0		_aSpecial Reviews -- The Temporal Sequence of Changes in Gene Expression by Drugs of Abuse -- Effects of Psychomotor Stimulants on Glutamate Receptor Expression -- Adult Neural Stem/Progenitor Cells in the Forebrain -- Neuroprotective Effect of Naloxone in Inflammation-Mediated Dopaminergic Neurodegeneration -- Neuropharmacology and Neurotoxicity of 3,4-Methylenedioxymethamphetamine -- Learning and Memory Mechanisms Involved in Compulsive Drug Use and Relapse -- From Drugs of Abuse to Parkinsonism: The MPTP Mouse Model of Parkinson's Disease -- Detection of MRNA Expression in the Striatum -- In Situ Hybridization with Isotopic Riboprobes for Detection of Striatal Neuropeptide mRNA Expression After Dopamine Stimulant Administration -- Combining In Situ Hybridization with Retrograde Tracing and Immunohistochemistry for Phenotypic Characterization of Individual Neurons -- Analysis of mRNA Expression Using Double In Situ Hybridization Labeling with Isotopic and Nonisotopic Probes -- Quantification of mRNA in Neuronal Tissue by Northern Analysis -- Analysis of Gene Expression in Striatal Tissue by Multiprobe RNase Protection Assay -- Analysis of mRNA Expression in Striatal Tissue by Differential Display Polymerase Chain Reaction -- Semiquantitative Real-Time PCR for Analysis of mRNA Levels -- Application of TaqMan RT-PCR for Real-Time Semiquantitative Analysis of Gene Expression in the Striatum -- Systematic Screening of Gene Expression Using a cDNA Macroarray -- Detection of Protein Expression in the Striatum -- Quantification of Protein in Brain Tissue by Western Immunoblot Analysis -- Immunohistochemical and Immunocytochemical Detection of Phosphoproteins in Striatal Neurons -- Analysis of Protein Expression in Brain Tissue by ELISA -- Dopamine Receptor Binding and Quantitative Autoradiographic Study -- Analysis of DNA-Binding Activity in Neuronal Tissue with the Electrophoretic Mobility-Shift Assay -- Gene Function Analysis -- Viral-Mediated Gene Transfer to Study the Behavioral Correlates of CREB Function in the Nucleus Accumbens of Rats -- Generating Gene Knockout Mice for Studying Mechanisms Underlying Drug Addiction -- Antisense Approaches in Analyzing the Functional Role of Proteins in the Central Nervous System -- Striatal Culture Preparation -- Primary Striatal Neuronal Culture -- Primary Rat Mesencephalic Neuron-Glia, Neuron-Enriched, Microglia-Enriched, and Astroglia-Enriched Cultures -- Primary Culture of Adult Neural Progenitors -- Organotypic Culture of Developing Striatum -- Detection of Transmitter Release in the Striatum -- Microdialysis Coupled with Electrochemical Detection -- HPLC/EC Detection and Quantification of Acetylcholine in Dialysates -- Real-Time Measurements of Phasic Changes in Extracellular Dopamine Concentration in Freely Moving Rats by Fast-Scan Cyclic Voltammetry -- Measurement of Dopamine Uptake in Neuronal Cells and Tissues -- Behvioral Assessment and Others -- Laboratory Analysis of Behavioral Effects of Drugs of Abuse in Rodents -- Conditioned Place Preference -- Detection of Cell Proliferation and Cell Fate in Adult CNS Using BrdU Double-Label Immunohistochemistry.
520			_aNew molecular technologies have transformed our understanding of the neurological impact of drugs of abuse and opened many new possibilities for research. In Drugs of Abuse: Neurological Reviews and Protocols, leading scientists from all over the world review these novel research areas and provide a cutting-edge collection of updated and core techniques for the neurological study of drugs of abuse. Described in step-by-step detail, these readily reproducible protocols cover a wide variety of coherent methods for gathering information on quantitative changes in protein and mRNA at both tissue and cellular levels. There are various methods for detecting single and multiple alterations in single and multiple gene expression, for analyzing the functional roles of genes and proteins, for studying the release kinetics of striatal dopamine, and for the quantitative measurement of such neurotransmitters as acetylcholine. Additional methods are provided for primary neural cell culture preparation, for an immunohistochemical method to examine cellular proliferation and differentiation, and for a culture method to grow viable neural progenitors. All contributors have considerable experience with the protocols covered, and each protocol has been thoroughly tried and successfully tested in the laboratory. Helpful notes for each method discuss tricks-of-the-trade, pitfalls to avoid, and troubleshooting. State-of-the-art and highly practical, Drugs of Abuse: Neurological Reviews and Protocols offers insightful reviews of key current topics and a core collection of readily reproducible techniques essential for any neurobiological laboratory carrying out either basic analyses or seeking novel research approaches.
700	1		_aWang, John Q _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617373282
776	0	8	_iPrinted edition: _z9781588290571
776	0	8	_iPrinted edition: _z9781489938466
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592593585 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2003
999			_c234012 _d234012