000			04529nam a22003735i 4500
001			234015
003			ES-VaUE
005			20221220020535.0
007			cr nn 008mamaa
008			100301s2004 xxu| s |||| 0|eng d
020			_a9781592594283
024	7		_a10.1385/159259428X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aBiopolymer Methods in Tissue Engineering _cedited by Anthony P. Hollander, Paul V. Hatton.
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XV, 257 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v238
505	0		_aProcessing of Resorbable Poly-?-Hydroxy Acids for Use as Tissue-Engineering Scaffolds -- Fibrin Microbeads (FMB) As Biodegradable Carriers for Culturing Cells and for Accelerating Wound Healing -- Synthesis and Characterization of Hyaluronan-Based Polymers for Tissue Engineering -- Synthesis and Characterization of Chitosan Scaffolds for Cartilage-Tissue Engineering -- Characterization of a Calcium Phosphate-Based Matrix for rhBMP-2 -- Methodologies for Processing Biodegradable and Natural Origin Scaffolds for Bone and Cartilage Tissue-Engineering Applications -- Alginates in Tissue Engineering -- Production and Surface Modification of Polylactide-Based Polymeric Scaffolds for Soft-Tissue Engineering -- Modification of Materials With Bioactive Peptides -- Isolation and Osteogenic Differentiation of Bone-Marrow Progenitor Cells for Application in Tissue Engineering -- Cell Seeding of Polymer Scaffolds -- Chondrocyte Isolation, Expansion, and Culture on Polymer Scaffolds -- Bioreactor Culture Techniques for Cartilage-Tissue Engineering -- Microscopic Methods for the Analysis of Engineered Tissues -- Transmission Electron Microscopy of Tissue-Polymer Constructs -- Application of Microscopic Methods for the Detection of Cell Attachment to Polymers -- Biochemical Methods for the Analysis of Tissue-Engineered Cartilage -- Real-Time Quantitative RT-PCR Assays -- Mechanical Testing of Cell-Material Constructs: A Review.
520			_aBecause the rapidly developing field of tissue engineering encompasses all the major scientific disciplines-including materials science, polymer chemistry, biology, and medicine-it has often been difficult to uncover the full range of laboratory methods required in tissue engineering. In Biopolymer Methods in Tissue Engineering, expert laboratory researchers bring together in a standard format all the diverse laboratory methods needed to perform state-of-the-art tissue engineering research. Topics range from the synthesis, processing, and characterization of specific biomaterials, through the successful use of scaffolds in the engineering of tissues, to techniques useful in evaluating the biological quality of scaffold-engineered tissues. Subjects of special interest include the incorporation of biological molecules into scaffold biomaterials and the use of a wide range of methods and techniques to generate a comprehensive description of cell-polymer construct quality. Additional techniques can be used to develop effective in vitro models for drug evaluation or to investigate cell and tissue differentiation. Each readily reproducible protocol follows a uniform format that includes essential background information, step-by-step instructions by an expert, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Biopolymer Methods in Tissue Engineering offers both advanced and novice investigators a diverse collection of readily reproducible techniques for generating living cell-polymer constructs in their laboratories and for evaluating their biological quality.
700	1		_aHollander, Anthony P _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aHatton, Paul V _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617372803
776	0	8	_iPrinted edition: _z9780896039674
776	0	8	_iPrinted edition: _z9781489939357
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259428X _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2004
999			_c234015 _d234015