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008			170102s2017 xxu| s |||| 0|eng d
020			_a9781493967346
024	7		_a10.1007/978-1-4939-6734-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMicrochip Diagnostics _bMethods and Protocols _cedited by Valérie Taly, Jean-Louis Viovy, Stéphanie Descroix.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 223 páginas) _b84 ilustraciones, 74 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1547
505	0		_aMicrofluidics-enabled Diagnostic Systems: Markets, Challenges, and Examples -- Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Efficient Fabrication and Sealing of Chips Using a "Chip-olate" Process -- Capillary-driven Microfluidic Chips for Miniaturized Immunoassays: Patterning Capture Antibodies using Microcontact Printing and Dry-film Resists -- Fabrication and Evaluation of Microfluidic Immunoassay Devices with Antibody-immobilized Microbeads Retained in Porous Hydrogel Micropillars -- Using Electrophoretic Immunoassay to Monitor Hormone Secretion -- On-chip Magnetic Particle-based Immunoassays using Multilaminar Flow for Clinical Diagnostic -- Digital Microfluidics Assisted Sealing of Individual Magnetic Particles in fFmtoliter-sized Reaction wells for Single-molecule Detection -- Microfluidic DNA Stretching Device for Single-Molecule Diagnostics -- Droplet Microfluidic and Magnetic Particles Platform for Cancer Typing -- SlipChip Device for Digital Nucleic Acid Amplification -- Multiplex Detection of KRAS Mutations using Passive Droplet Fusion -- Droplet-based Microfluidics Digital PCR for the Detection of KRAS Mutations -- Negative Enrichment of Circulating Tumor Cells in Blood Using a Microfluidic Chip -- Microfluidic Based Bacteria Isolation from Whole Blood for Diagnostics of Blood Stream Infection -- Microfluidic POlatform for Parallel Single Cell Analysis for Diagnostic Applications -- FISH-in-CHIPS. .
520			_aThis volume illustrates how microfluidic approaches can meet the requirement of clinical diagnosis based on molecular or cellular biomarkers. Microchip Diagnostics: Methods and Protocols is divided into four sections describing the business aspects of the microfluidic-based systems for diagnosis, demonstrating how versatile microfluidics can be regarding to protein bioassay integration, presenting microfluidic approaches for nucleic analysis based on mono or diphasic format, and highlighting recent contributions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
700	1		_aTaly, Valérie _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aViovy, Jean-Louis _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aDescroix, Stéphanie _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493967322
776	0	8	_iPrinted edition: _z9781493967339
776	0	8	_iPrinted edition: _z9781493982868
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6734-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234024 _d234024