000			04260nam a22003615i 4500
001			234026
003			ES-VaUE
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007			cr nn 008mamaa
008			130804s2013 xxu| s |||| 0|eng d
020			_a9781627035651
024	7		_a10.1007/978-1-62703-565-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDNA Electrophoresis _bMethods and Protocols _cedited by Svetlana Makovets.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (X, 299 páginas) _b56 ilustraciones, 20 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1054
505	0		_aDNA Electrophoresis: Historical and Theoretical Perspectives -- Basic DNA Electrophoresis in Molecular Cloning: a Comprehensive Guide for Beginners -- Analysis of Branched DNA Replication and Recombination Intermediates from Prokaryotic Cells by Two-dimensional (2D) Native-native Agarose Gel Electrophoresis -- Analysis of DNA Structures from Eukaryotic cells by Two-dimensional Native-native DNA Agarose Gel Electrophoresis -- Directionality of Replication Fork Movement Determined by Two-dimensional Native-native DNA Agarose Gel Electrophoresis -- Native/Denaturing Two-Dimensional DNA Electrophoresis And Its Application To The Analysis Of Recombination Intermediates -- Plasmid DNA Topology Assayed by Two-dimensional Agarose Gel Electrophoresis -- A Neutral Glyoxal Gel Electrophoresis Method for the Detection and Semi-Quantitation of DNA Single-Strand Breaks -- Denaturing Gradient Gel Electrophoresis (DGGE) -- Polyacrylamide Temperature Gradient Gel Electrophoresis -- Separation of DNA Oligonucleotides using Denaturing Urea-PAGE -- Pulsed-field Gel Electrophoresis of Bacterial Chromosomes -- Resolution of Budding Yeast Chromosomes using Pulse Field Gel Electrophoresis -- Analysis of DNA Damage Via Single Cell Electrophoresis -- Fluorescence in situ Hybridisation on Electrophoresed Cells to Detect Sequence Specific DNA Damage -- Analysis of Protein-DNA Interactions using PAGE: Band-shift Assays -- Assaying Cooperativity of Protein-DNA Interactions using Agarose Gel Electrophoresis -- DNA Bending by Proteins: Utilizing Plasmid pBendAT as a Tool -- Using PCR coupled to PAGE for Detection and Semi-quantitative Evaluation of Telomerase Activity.
520			_aIn DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.
700	1		_aMakovets, Svetlana _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627035668
776	0	8	_iPrinted edition: _z9781627035644
776	0	8	_iPrinted edition: _z9781493959983
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-565-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c234026 _d234026