000			04009nam a22003615i 4500
001			234045
003			ES-VaUE
005			20221220020537.0
007			cr nn 008mamaa
008			180628s2018 xxu| s |||| 0|eng d
020			_a9781493985678
024	7		_a10.1007/978-1-4939-8567-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHandbook of ELISPOT _bMethods and Protocols _cedited by Alexander E. Kalyuzhny.
250			_a3rd edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (X, 251 páginas) _b74 ilustraciones, 68 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1808
505	0		_aChallenges in Developing Protein Secretion Assays at a Single-Cell Level -- To Count of Not to Count: ELISPOT Evaluation Revisited -- Essential Controls for ELISpot Assay -- Automatic Search of Spots and Color Classification in ELISPOT Assay -- Four Color ImmunoSpot ® Assays for Identification of Effector T Cell Lineages -- Detection of Cross-Reactive B Cells Using the FluoroSpot Assay -- Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations -- Detecting All Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® Assays -- Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpot® Software Based on Center of Mass Distance Algorithm -- Multi-Color FLUOROSPOT Counting Using ImmunoSpot® Fluoro-X™ Suite -- B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Blood Mononuclear Cells -- Identification of Novel Mycobacterial Targets for Murine CD4+T Cells by IFN ELISPOT -- ELISPOT-Based "Multi-Color FluoroSpot" to Study Type-Specific and Cross-Reactive Responses in Memory B Cells after Dengue and Zika Virus Infections -- Cultured ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses -- Ex Vivo ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses -- Ex Vivo ELISpot Assay to Investigate iNKT Cell Responses in Acute Dengue Infection -- Dendritic Cell-Based ELISpot Assay for Assessing T Cell-IFN- γ Responses in Human Peripheral Blood Mononuclear Cells to Dengue Envelope Proteins -- Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccines -- Detection and Quantification of Influenza A/H1N1 Virus-Specific Memory B Cells in Human PBMCs using ELISpot Assay.
520			_aThis third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot's sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research, essentials control, and assay evaluation in ELISpot. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Handbook of ELISPOT: Methods and Protocols, Third Edition is a valuable resource for both novice researchers and experienced ELISpot users.
700	1		_aKalyuzhny, Alexander E _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493985661
776	0	8	_iPrinted edition: _z9781493985685
776	0	8	_iPrinted edition: _z9781493993284
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8567-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c234045 _d234045