000			03566nam a22003615i 4500
001			234048
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007			cr nn 008mamaa
008			170531s2017 xxu| s |||| 0|eng d
020			_a9781493970636
024	7		_a10.1007/978-1-4939-7063-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Receptor Kinases _bMethods and Protocols _cedited by Reidunn Birgitta Aalen.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XII, 196 páginas) _b41 ilustraciones, 18 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1621
505	0		_aExpression of Plant Receptor Kinases in E. coli -- Expression of Plant Receptor Kinases in Yeast -- Expression of Plant Receptor Kinases in Tobacco BY-2 Cells -- Cell-free Synthesis of Plant Receptor Kinases -- Purification of Plant Receptor Kinases from Plant Plasma Membranes -- Photoaffinity Labeling of Plant Receptor Kinases -- Quantitative Detection of Oxidative Burst upon Activation of Plant Receptor Kinases -- Extraction and Curation of Gene Models for Plant Receptor Kinases for Phylogenetic Analysis -- In silico Prediction of Ligand Binding Sites of Plant Receptor Kinases using Conservation Mapping -- Co-immunoprecipitation of Plant Receptor Kinases -- Autophosphorylation Assays using Plant Receptor Kinases Synthesized in Cell-free Systems -- Investigation of Autophosphorylation Sites of Plant Receptor Kinases and Phosphorylation of Interacting Partners -- In vitro Assessment of Guanylyl Cyclase Activity of Plant Receptor Kinases -- BiFC Assay to Detect Calmodulin Binding to Plant Receptor Kinases -- Pull Down Assay to Characterize Ca2+/Calmodulin Binding to Plant Receptor Kinases -- The use of FRET/FLIM to Study Proteins Interacting with Plant Receptor Kinases -- Detection and Analyses of Endocytosis of Plant Receptor Kinases.
520			_aThis is volume presents protocols relevant for plant receptor kinases(PRK) both in development and defence. Chapters guide readers through comprehensive experimental approach for molecular investigations of plant receptor kinases, from the simplest methods for expression and purification of receptor domains to the most advanced methods aiming at understanding the dynamics of receptor complex formation and specificity of signaling pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Receptor Kinases: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aAalen, Reidunn Birgitta _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493970629
776	0	8	_iPrinted edition: _z9781493970643
776	0	8	_iPrinted edition: _z9781493983827
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7063-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234048 _d234048