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020 _a9781493967285
024 7 _a10.1007/978-1-4939-6728-5
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aExosomes and Microvesicles
_bMethods and Protocols
_cedited by Andrew F Hill.
250 _a1st edition 2017
264 1 _aNew York, NY
_bSpringer International Publishing
_c2017
300 _a1 recurso en línea (XII, 228 páginas)
_b39 ilustraciones, 21 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1545
505 0 _aMethods to Analyze EVs -- Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles -- Immuno-Characterization of Exosomes using Nanoparticle Tracking Analysis -- Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy -- Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR -- Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM™ System -- A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration -- Multiplexed Phenotyping of Extracellular Vesicles using Protein Microarray (EV Array) -- Purification and Analysis of Exosomes Released by Mature Cortical Neurons following Synaptic Activation -- A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space -- Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission -- Isolation of Platelet-Derived Extracellular Vesicles -- Bioinformatics Tools for Extracellular Vesicles Research -- Preparation and Isolation of siRNA-Loaded Extracellular Vesicles -- Interaction of Extracellular Vesicles with Endothelial Cells under Physiological Flow Conditions.
520 _aThis volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing proteins and genomic materials using a number of different approaches, and isolating EVs from platelets and neuronal cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensive, Exosomes and Microvesicles: Methods and Protocols is a valuable resource containing methodologies for anyone interested in researching EVs.
700 1 _aHill, Andrew F
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493967261
776 0 8 _iPrinted edition:
_z9781493967278
776 0 8 _iPrinted edition:
_z9781493982844
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6728-5
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2017
999 _c234078
_d234078