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008			130614s2013 xxu| s |||| 0|eng d
020			_a9781461472094
024	7		_a10.1007/978-1-4614-7209-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Low Molecular Weight Proteome _bMethods and Protocols _cedited by Helena Bäckvall, Janne Lehtiö.
250			_a1st edition 2013
264		1	_aNew York, NY _bSpringer International Publishing : _bImprint: Springer, _c2013
300			_a1 recurso en línea (XII, 223 páginas) _b50 ilustraciones, 26 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1023
505	0		_aNarrow Range Peptide Iso Electric Focusing as Peptide Prefractionation Method Prior to Tandem Mass Spectrometry Analysis -- Analysis of Peptides by Denaturing Ultrafiltration and LC-MALDI-TOF-MS -- Stable Isotope Labeling Methods in Protein Profiling -- Development of MRM-based Assays for the Absolute Quantitation of Plasma Proteins -- Mass Spectrometric Profiling of Low-Molecular-Weight Proteins -- Developing an iMALDI Method -- Analysis of Neuropeptides by MALDI Imaging Mass Spectrometry -- Highly Multiplexed Antibody Suspension Bead Arrays for Plasma Protein Profiling -- Protein Quantification by Peptide Quality Control (PQPQ) of Shotgun Proteomics Data -- Collection and Handling of Blood Specimens for Peptidomics -- An Automated RP-SCX Solid Phase Extraction Procedure for Urinary Peptidomics Biomarker Discovery Studies -- Application of Phage Display for Ligand Peptidomics to Identify Peptide Ligands Binding to AQP2-expressing Membrane Fractions -- Protein Expression Profiling of Brain Tumor Tissue Using SELDI-MS -- Comprehensive Analysis of MHC Ligands in Clinical Material by Immunoaffinity - Mass Spectrometry.
520			_aSmall proteins with molecular weights of <25 kDa are involved in major biological processes such as ribosome formation, stress  adaption and cell cycle control. The study of the low-molecular-weight proteome has identified many central regulators of biology such as cytokines, chemokines, peptide hormones and proteolytic fragments of larger proteins. Due to the unique features of these proteins, the technical challenges are different from those in "common" proteomics.  In The Low Molecular Weight Proteome: Methods and Protocols expert researchers from the field provide protocols for analysis of low molecular weight proteins and peptides, protocols for such methods applied in clinical research and an up-to-date review of quantitative protein profiling by labeling.  These include methods suitable for both peptide and protein analysis with focus on methods and application that can be used for small protein analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, The Low Molecular Weight Proteome: Methods and Protocolsis a useful resource for experienced proteomics practitioners as well as an aid to newcomers who wish to become acquainted with the theory and practice of a wide array of methods in analyzing small proteins or peptides.
700	1		_aBäckvall, Helena _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aLehtiö, Janne _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781461472100
776	0	8	_iPrinted edition: _z9781461471677
776	0	8	_iPrinted edition: _z9781493955671
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4614-7209-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c234093 _d234093