000			04115nam a22003735i 4500
001			234097
003			ES-VaUE
005			20221220020540.0
007			cr nn 008mamaa
008			160922s2016 xxu| s |||| 0|eng d
020			_a9781493963805
024	7		_a10.1007/978-1-4939-6380-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPolycomb Group Proteins _bMethods and Protocols _cedited by Chiara Lanzuolo, Beatrice Bodega.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XV, 325 páginas) _b40 ilustraciones, 23 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1480
505	0		_aMapping the Function of Polycomb Proteins -- Chromatin Immunoprecipitation -- Chromatin preparation and Chromatin Immuno-Precipitation from Drosophila embryos -- ChIP-seq data processing for PcG proteins and associated histone modifications -- Analysis of single locus replication timing in asynchronous cycling cells -- Non coding RNA interplay with the genome -- RIP: RNA Immunoprecipitation -- Capture Hybridization Analysis of DNA Targets -- Identification of RNA-protein interactions through in-vitro RNA pull-down assays -- Understanding RNA-chromatin interactions using Chromatin Isolation by RNA Purification (ChIRP) -- Analysis RNA-seq and non coding RNA -- The dynamics of Polycomb complexes -- Fluorescence resonance energy transfer microscopy for measuring chromatin complex structure and dynamics -- Analysis of endogenous protein interactions of Polycomb-group of proteins in mouse embryonic stem cells -- Determination of Polycomb group of proteins compartmentalization through chromatin fractionation procedure -- An Automatic Segmentation Method Combining an Active Contour Model and a Classification Technique for Detecting Polycomb-group Proteins in High-Throughput Microscopy Images -- Polymer physics of the large-scale structure of chromatin -- Chromosome Conformation Capture in Drosophila -- Chromosome Conformation Capture in primary human cells -- Determination of high-resolution 3D chromatin organization using Circular Chromosome Conformation Capture (4C-seq) -- Chromosome Conformation Capture on Chip (4C): Data Processing -- In vivo models to address the function of Polycomb Group Proteins -- A rapid TALEN assembly protocol -- Following the motion of Polycomb bodies in living Drosophila embryos -- Reprogramming of Somatic Cells towards Pluripotency by Cell Fusion -- Imaginal discs transplantation in Drosophila -- Isolation and culture of muscle stem cells. .
520			_aThis volume presents the most recent technologies used in the Polycomb Group Proteins (PcG) field. Chapters detail state-of-the-art methods, creating a unique and comprehensive reference source for investigating Polycomb function in the nucleus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Polycomb Group Proteins: Method and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aLanzuolo, Chiara _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBodega, Beatrice _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493963782
776	0	8	_iPrinted edition: _z9781493963799
776	0	8	_iPrinted edition: _z9781493981816
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6380-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234097 _d234097