000			04508nam a22003615i 4500
001			234100
003			ES-VaUE
005			20221220020541.0
007			cr nn 008mamaa
008			140417s2014 xxu| s |||| 0|eng d
020			_a9781493905638
024	7		_a10.1007/978-1-4939-0563-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aYeast Metabolic Engineering _bMethods and Protocols _cedited by Valeria Mapelli.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (X, 316 páginas) _b44 ilustraciones, 18 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1152
505	0		_aAn Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae -- Natural and Modified Promoters for Tailored Metabolic Engineering of the Yeast Saccharomyces cerevisiae -- Tools for Genetic Engineering of the Yeast Hansenula polymorpha -- Molecular Tools and Protocols for Engineering the Acid Tolerant Yeast Zygosaccharomyces bailii as a Potential Cell Factory -- Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris -- Methods for Efficient High Throughput Screening of Protein Expression in Recombinant Pichia pastoris Strains -- Synthetic RNA Switches for Yeast Metabolic Engineering: Screening Recombinant Enzyme Libraries -- Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology -- Genetic Engineering of Industrial Saccharomyces cerevisiae Strains Using a Selection/Counter-Selection Approach -- Evolutionary Engineering of Yeast -- Determination of a Dynamic Feeding Strategy for Recombinant Pichia pastoris Strains -- Yeast Metabolomics: Sample Preparation for a GC/MS-Based Analysis.- 13C-Based Metabolic Flux Analysis in Yeast: The Pichia pastoris Case -- Pathway Activity Profiling (PAPi): A Tool for Metabolic Pathway Analysis -- QTL Mapping by Pooled-Segregant Whole Genome Sequencing in Yeast -- Genome Scale Metabolic Models of Yeast, Methods for Their Reconstruction and Other Applications -- Model-Guided Identification of Gene Deletion Targets for Metabolic Engineering in Saccharomyces cerevisiae -- Patents: A Tool to Bring Innovation from the Lab Bench to the Marketplace.
520			_aYeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha, and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail, as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume.  Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.
700	1		_aMapelli, Valeria _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493905645
776	0	8	_iPrinted edition: _z9781493905621
776	0	8	_iPrinted edition: _z9781493952861
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0563-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c234100 _d234100