000			04119nam a22003735i 4500
001			234104
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007			cr nn 008mamaa
008			100301s2009 xxu| s |||| 0|eng d
020			_a9781597453523
024	7		_a10.1007/978-1-59745-352-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aKidney Research _bExperimental Protocols _cedited by Tim D. Hewitson, Gavin J. Becker.
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XVI, 272 páginas) _b62 ilustraciones, 10 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v466
505	0		_aIsolation and Propagation of Cell Populations -- Isolation and Propagation of Glomerular Mesangial Cells -- Isolation and Primary Culture of Human Proximal Tubule Cells -- Propagation and Culture of Renal Fibroblasts -- Animal Models -- Small Animal Models of Kidney Disease: A Review -- Techniques and Applications -- Measurement of Glomerular Filtration Rate in Conscious Mice -- Laser Capture Microdissection of Kidney Tissue -- Quantitative Gene Expression Analysis in Kidney Tissues -- In Vivo Imaging of Leukocyte Recruitment to Glomeruli in Mice Using Intravital Microscopy -- Using In Situ Hybridisation to Localize Renal Gene Expression in Tissue Sections -- Immuno and Lectin Histochemistry for Renal Light Microscopy -- Immuno and Lectin Histochemistry for Renal Electron Microscopy -- Pimonidazole Adduct Immunohistochemistry in the Rat Kidney: Detection of Tissue Hypoxia -- Identification of Apoptosis in Kidney Tissue Sections -- Cell-Populated Floating Collagen Lattices: An In Vitro Model of Parenchymal Contraction -- Mechanical Stretch-Induced Signal Transduction in Cultured Mesangial Cells -- Determination of Collagen Content, Concentration, and Sub-types in Kidney Tissue -- SELDI-TOF Mass Spectrometry-Based Protein Profiling of Kidney Tissue -- In Vivo Transfer of Small Interfering RNA or Small Hairpin RNA Targeting Glomeruli.
520			_aAs the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment. The study of renal disease, however, suffers due to the complex nature of renal anatomy and physiology and the plethora of different cell types found in the kidney. In Kidney Research: Experimental Protocols, top experts in the field seek to aid researchers by providing a number of specialized techniques developed to examine this intricate system. Through both well-established and novel methods, this volume explores the preparation and culture of the main cell types used to study renal disease mechanisms, the common animal models used to mimic the various forms of human renal disease, and specific applications and techniques used in vivo and in vitro. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls. Essential and cutting-edge, Kidney Research: Experimental Protocols delivers invaluable guidance to new and experienced laboratory researchers delving into the sophisticated study of the kidney.
700	1		_aHewitson, Tim D _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBecker, Gavin J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607610717
776	0	8	_iPrinted edition: _z9781588299451
776	0	8	_iPrinted edition: _z9781493956401
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-352-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c234104 _d234104