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001			234107
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008			100301s2001 xxu| s |||| 0|eng d
020			_a9781592591435
024	7		_a10.1385/1592591434 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aAngiogenesis Protocols _cedited by J. Clifford Murray.
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (X, 267 páginas) _b119 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v46
505	0		_aSpecial Review Article -- Therapeutic Inhibition of Angiogenesis -- Angiogenesis Protocols in Vivo -- Microscopic Assessment of Angiogenesis in Tumors -- In Vivo Matrigel Migration and Angiogenesis Assays -- Alginate Microbead Release Assay of Angiogenesis -- Disc Angiogenesis Assay -- Sponge Implant Model of Angiogenesis -- Hollow Fiber Assay for Tumor Angiogenesis -- Dorsal Skinfold Chamber Preparation in Mice -- Angiogenesis Assays Using Chick Chorioallantoic Membrane -- Corneal Assay for Angiogenesis -- Angiogenesis Protocols in Vitro -- Collagen Gel Assay for Angiogenesis -- Chemotaxis and Chemokinesis in 3D Macromolecular Matrices -- Rat Aortic Ring -- In Vitro Matrigel Angiogenesis Assays -- Associated Techniques -- Microvessel Endothelial Cells from Human Adipose Tissues -- Transfection and Transduction of Primary Human Endothelial Cells -- Vascular Smooth Muscle Cells -- Bovine Retinal Microvascular Pericytes.
520			_aAlthough angiogenesis-the formation of new blood vessels from pre-existing ones-has emerged as an exciting new target for the treatment of many varied disorders, fundamental aspects of this important biological process remain poorly understood. To address the needs of those working in such a dramatically advancing area, Cliff Murray and a panel of experienced investigators have brought together in Angiogenesis Protocols a comprehensive collection of key techniques for assessing the formation of new blood vessels in both whole tissues and cell culture. These readily reproducible methods range from low-cost in vitro assays to cutting-edge in vivo models, such as the dorsal window chamber, which demands high levels of surgical skill as well as relatively expensive, custom-made equipment. The authors have also included details of the associated techniques for the isolation, identification, and culture of microvessel endothelial cells from human adipose tissues and for the transfection and transduction of primary human endothelial cells. Timely and highly practical, Angiogenesis Protocols is a gold-standard resource for today's angiogenesis investigator, providing easy access to a wide variety of proven assays suitable for laboratories with greatly differing technical expertise, materials, and financial resources.
700	1		_aMurray, J. Clifford _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489942340
776	0	8	_iPrinted edition: _z9781489942333
776	0	8	_iPrinted edition: _z9780896036987
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591434 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2001
999			_c234107 _d234107