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020 _a9781592599660
024 7 _a10.1385/1592599664
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlant-Pathogen Interactions
_cedited by Pamela C. Ronald.
250 _a1st edition 2007
264 1 _aTotowa, NJ
_bHumana Press
_c2007
300 _a1 recurso en línea (XII, 248 páginas)
_b24 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v354
505 0 _aThe Use of Protoplasts to Study Innate Immune Responses -- Marker-Exchange Mutagenesis and Complementation Strategies for the Gram-Negative Bacteria Xanthomonas oryzae pv. oryzae -- Whole-Genome Analysis to Identify Type III-Secreted Effectors -- In planta Expression of Oomycete and Fungal Genes -- Use of Nipponbare BAC Clones for Physical Mapping of an R Gene Locus in Rice -- Agrobacterium-Mediated Transformation to Create an Insertion Library in Magnaporthe grisea -- Identification of Components in Disease-Resistance Signaling in Arabidopsis by Map-Based Cloning -- Yeast Two-Hybrid Approaches to Dissecting the Plant Defense Response -- Use of Rolling-Circle Amplification for Large-Scale Yeast Two-Hybrid Analyses -- Preparative Denaturing Isoelectric Focusing for Enhancing Sensitivity of Proteomic Studies -- Use of Massively Parallel Signature Sequencing to Study Genes Expressed During the Plant Defense Response -- Use of Microarray Analysis to Dissect the Plant Defense Response -- Use of Robust-Long Serial Analysis of Gene Expression to Identify Novel Fungal and Plant Genes Involved in Host-Pathogen Interactions -- Analysis of Gene Function in Rice Through Virus-Induced Gene Silencing -- Use of RNA Interference to Dissect Defense-Signaling Pathways in Rice -- Virus-Induced Gene Silencing in Plant Roots -- Methods for Engineering Resistance to Plant Viruses -- Development of Genetically Engineered Resistant Papaya for papaya ringspot virus in a Timely Manner.
520 _aPlant-Pathogen Interactions: Methods and Protocols provides key methods, approaches, and strategies to dissect the plant defense response. Addressing methods to identify and characterize plant resistance genes as well as pathogen-associated molecules that trigger the plant defense response, this volume creates a better understanding of the interactions between pathogens and their hosts, which will help to develop better methods for disease control in plants and animals. Plant-Pathogen Interactions: Methods and Protocols reviews methods for engineering resistance to plant viruses, the utility of viral induced gene silencing and RNAi silencing, as well as advances in genomics and proteomics that have lead to new methods to identify genes and proteins. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and the avoidance of known pitfalls.
700 1 _aRonald, Pamela C
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617375767
776 0 8 _iPrinted edition:
_z9781588294487
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592599664
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2007
999 _c234118
_d234118