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020			_a9781592590728
024	7		_a10.1385/1592590721 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aAsthma _cedited by K. Fan Chung, Ian Adcock.
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (XI, 346 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v44
505	0		_aAsthma: Application of Cell and Molecular Biology Techniques to Unravel Causes and Pathophysiological Mechanisms -- Culture of Normal Human Airway Epithelial Cells and Measurement of Mucin Synthesis and Secretion -- Brush Biopsy and Culture of Airway Epithelial Cells: ?-Adrenergic Receptor System Function -- Isolation and Characterization of Human Airway Fibroblasts in Culture -- Flow Cytometric Analysis of Blood Monocytes and Alveolar Macrophages -- Expression of IL-10 and GM-CSF in Blood Monocytes and Alveolar Macrophages -- MAP Kinase Expression in Eosinophils -- Human Eosinophil Isolation and the Measurement of Apoptosis -- Adhesion of T-Cells to Airway Smooth Muscle Cells -- Proliferation of Circulating T-Cells -- Differential Display Analysis of Inflammatory Genes in Airway Epithelium -- Analysis of Transcription Factor Activation: NF?B as a Regulator of Inflammatory Genes in Epithelial Cells -- Transient Transgenic Approaches for Investigating the Role of GM-CSF in Pulmonary Inflammation and Immune Diseases -- Quantitative Analysis of RANTES mRNA in Endobronchial Biopsies Using Polymerase Chain Reaction -- Intracellular Cytokine Staining for Analysis by Flow Cytometry -- Localization of IL-4 and IL-5 mRNA in Bronchial Biopsies -- Immunohistochemical Analysis of Adhesion Molecules in Airway Biopsies -- Site-Directed Mutagenesis in the Investigation of ?-Adrenoreceptor Exosite -- Methods for Determining ?2-Adrenoreceptor Genotype -- Modulation of ?-Adrenoreceptor Expression in the Airways -- Chemical Mutational Analysis of Glucocorticoid Receptor -- Histone Acetylation and Deacetylation -- Genome-Wide Search for Asthma Genes.
520			_aAs asthma increases in prevalence and severity around the world, the need for understanding its basic mechanisms and for developing better drugs and preventative measures becomes more urgent. In Asthma: Mechanisms and Protocols, K. Fan Chung and Ian Adcock bring together the first collection of laboratory methods for studying the molecular and cellular mechanisms of asthma. Leading experts describe highly effective methods for obtaining cells from asthmatic airways, analyzing gene and protein expression in these clinical samples, and using molecular and cellular tools to study cytokine expression and release in this disease. There are also cutting-edge techniques for studying asthma-related genes and genetic polymorphisms, and for understanding the effects of asthma treatment. User-friendly and state-of-the-art, Asthma: Mechanisms and Protocols provides all asthma researchers-whether novice or experienced, whether in experimental or clinical research-with a first-class collection of readily reproducible pharmacological, cellular, molecular, biochemical, and genetic methods for elucidating not only the mechanisms of the disease itself, but also of the drugs for asthma's treatment.
700	1		_aChung, K. Fan _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aAdcock, Ian _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617371035
776	0	8	_iPrinted edition: _z9780896036260
776	0	8	_iPrinted edition: _z9781489941480
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590721 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2000
999			_c234119 _d234119