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001			234124
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007			cr nn 008mamaa
008			100301s2000 xxu| s |||| 0|eng d
020			_a9781592590490
024	7		_a10.1385/1592590497 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
100	1		_aCrowther, John R. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
245	1	4	_aThe ELISA Guidebook _cby John R. Crowther.
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (XI, 421 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v149
505	0		_aOverview of ELISA in Relation to Other Disciplines -- Systems in ELISA -- Stages in ELISA -- Titration of Reagents -- Theoretical Considerations -- Practical Exercises -- Monoclonal Antibodies -- Validation of Diagnostic Tests for Infectious Diseases -- Charting Methods for Internal Quality Control -- Immunochemical Techniques -- Test Questions.
520			_aHighly sensitive, precise, and versatile, Enzyme Linked Immunosorbent Assay (ELISA) is now the predominant assay used in biomedical and agricultural research, as well as in applied science. In The ELISA Guidebook, John R. Crowther provides todayís premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. The author describes each method in great detail to ensure experimental success and includes advice on equipment choice, maintenance, and calibration. Wherever possible, helpful written explanations are provided along with copious diagrams. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
776	0	8	_iPrinted edition: _z9780896037281
776	0	8	_iPrinted edition: _z9781489938695
776	0	8	_iPrinted edition: _z9780896039506
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590497 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2000
999			_c234124 _d234124