000			03707nam a22003735i 4500
001			234128
003			ES-VaUE
005			20221220020542.0
007			cr nn 008mamaa
008			140224s2014 xxu| s |||| 0|eng d
020			_a9781493903573
024	7		_a10.1007/978-1-4939-0357-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCaspases,Paracaspases, and Metacaspases _bMethods and Protocols _cedited by Peter V. Bozhkov, Guy Salvesen.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XI, 266 páginas) _b66 ilustraciones, 34 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1133
505	0		_aGeneral in vitro Caspase Assay Procedures -- Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Specificity.-  Global Identification of Caspase Substrates using PROTOMAP (PROtein TOpography and Migration Analysis Platform) -- Caspase-2 Protocols -- Caspase-14 Protocols -- Caspase Protocols in Caenorhabditis elegans -- Detecting Caspase Activity in Drosophila Larval Imaginal Discs -- Methods for the Study of Caspase Activation in the Xenopus laevis Oocyte and Egg Extract -- Caspase Protocols in Mice -- Measurement of Caspase Activation in Mammalian Cell Cultures -- Detection and Measurement of Paracaspase MALT1 Activity -- Leishmania Metacaspase: an Arginine-Specific Peptidase -- Purification, Characterization, and Crystallisation of Trypanosoma Metacaspases -- Monitoring the Proteostasis Function of the Saccharomyces cerevisiae Metacaspase Yca1 -- Plant Metacaspase Activation and Activity -- Preparation of Arabidopsis thaliana Seedling Proteomes for Identifying Metacaspase Substrates by N-terminal COFRADIC.
520			_aCaspases, Paracaspases, and Metacaspacses: Methods and Protocols is  a collection of laboratory protocols covering current methods that are employed to measure and detect activities of these proteases in diverse biological systems, ranging from unicellular organisms to mammals.   Broken into two parts, the first part focuses on methods to measure, detect, and inhibit activation and activity of a subset of or specific caspases in vitro and in several model systems and organisms, primarily in the context of programmed cell death.  The second part of the book provides experimental protocols for purification and in vitro and in vivo analysis of yeast, protozoan and plant metacaspases, as well as of a human paracaspase MALT1. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.   Authoritative and practical, Caspases, Paracaspases, and Metacaspacses: Methods and Protocols seeks to aid scientists easy-to-follow techniques.
700	1		_aV. Bozhkov, Peter _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSalvesen, Guy _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493903580
776	0	8	_iPrinted edition: _z9781493903566
776	0	8	_iPrinted edition: _z9781493954056
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0357-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c234128 _d234128