000			03872nam a22003735i 4500
001			234132
003			ES-VaUE
005			20221220020543.0
007			cr nn 008mamaa
008			171013s2018 xxu| s |||| 0|eng d
020			_a9781493973392
024	7		_a10.1007/978-1-4939-7339-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aArgonaute Proteins _bMethods and Protocols _cedited by Katsutomo Okamura, Kotaro Nakanishi.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XIII, 260 páginas) _b55 ilustraciones, 44 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1680
505	0		_aCloning and Identification of Recombinant-Argonaute Bound Small RNAs using Next-Generation Sequencing -- Quantification of miRNAs Co-Immunoprecipitated with Argonaute Proteins using SYBR Green-Based qRT-PCR -- Gateway to Understanding Argonaute Loading of Single-Stranded RNAs: Preparation of Deep Sequencing Libraries with In Vitro Loading Samples -- Dumbbell-PCR for Discriminative Quantification of a Small RNA Variant -- MicroRNA Detection by Whole-Mount In Situ Hybridization in C. elegans -- cCLIP-Seq: Retrieval of Chimaeric Reads from HITS-CLIP (CLIP-Seq) Libraries -- Kinetic Analysis of Small Silencing RNA Production by Human and Drosophila Dicer Enzymes In Vitro -- Nucleic Acid-Binding Assay of Argonaute Protein using Fluorescence Polarization -- Reconstitution of RNA Interference Machinery -- Single-Molecule Analysis for RISC Assembly and Target Cleavage -- Profiling Open Chromatin Structure in the Ovarian Somatic Cells using ATAC-Seq -- Assessing miR-451 Activity and Its Role in Erythropoiesis -- Functional Analysis of MicroRNAs in Neurogenesis during Mouse Cortical Development -- Cellular Approaches in Investigating Argonaute2-Dependent RNA Silencing -- Genomic Tagging of AG01 using CRISPR/Cas9-Mediated Homologous Recombination -- Accurate Profiling and Quantification of tRNA Fragments from RNA-Seq Data: A vade mecum for MINTmap.
520			_aThis volume covers methods that analyze various Argonaute proteins from a variety of organisms to help researchers better understand their properties ranging from a molecular level to an organismal level. The chapters in this book explore the following topics: identification and expression analysis of guide nucleic acids and their targets; analysis of biochemical properties of Argonautes; biological functions of Argonautes; and obtaining materials and setting up analysis platforms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Argonaute Proteins: Methods and Protocols is a valuable resource for researchers and scientists looking to expand their knowledge of Argonaute proteins and their functions.
700	1		_aOkamura, Katsutomo _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aNakanishi, Kotaro _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493973385
776	0	8	_iPrinted edition: _z9781493973408
776	0	8	_iPrinted edition: _z9781493984572
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7339-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c234132 _d234132