000			04215nam a22003735i 4500
001			234139
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005			20221220020543.0
007			cr nn 008mamaa
008			180216s2018 xxu| s |||| 0|eng d
020			_a9781493976980
024	7		_a10.1007/978-1-4939-7698-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSertoli Cells _bMethods and Protocols _cedited by Marco G. Alves, Pedro F. Oliveira.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XIII, 297 páginas) _b49 ilustraciones, 37 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1748
505	0		_aEstablishment of Primary Culture of Sertoli Cells -- Evaluation of the Purity of Sertoli Cell Primary Cultures -- Preparation of Testicular Samples for Histology and Immunohistochemistry -- Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age -- Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy -- Sertoli Cells Preparation for Co-Immunoprecipitation -- Profiling Signaling Proteins in Sertoli Cells by Co-Immunoprecipitation -- Phagocytosis by Sertoli Cells: Analysis of Main Phagocytosis Steps by Confocal and Electron Microscopy -- A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells -- Assessing Autophagy in Sertoli Cells -- Molecular Mechanisms and Signaling Pathways Involved in the Nutritional Support of Spermatogenesis by Sertoli Cells -- Assessing Sertoli Cells Metabolic Activity -- Proteome Profiling of Sertoli Cells Using a GeLC-MS/MS Strategy -- Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs -- Testicular Cell Selective Ablation Using Diphtheria Toxin Receptor Transgenic Mice -- Regulation of Blood-Testis Barrier (BTB) Dynamics, Role of the Actin, and Microtubule-Based Cytoskeletons -- Monitoring the Integrity of the Blood-Testis Barrier (BTB): An In Vivo Assay -- Computational Methods Involved in Evaluating the Toxicity of the Reproductive Roxicants in Sertoli Cell -- A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sertoli Cells -- Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing.
520			_aThis detailed volume explores the use of Sertoli cells as models in male reproductive biology or as supporters for other cell types, and it answers the need for standardized protocols for their in vitro culture. Prepared for non-specialists as well as experienced researchers that may be interested in a multidisciplinary approach to study these cells, the book examines these remarkable cells that present some unique features that go far beyond the physical and nutritional support of spermatogenesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Sertoli Cells: Methods and Protocols serves as a valuable resource for experienced researchers in reproductive biology, particularly andrologists, but also for future investigators and young students starting to study these extraordinary cells.
700	1		_aAlves, Marco G _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aOliveira, Pedro F _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493976973
776	0	8	_iPrinted edition: _z9781493976997
776	0	8	_iPrinted edition: _z9781493985432
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7698-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c234139 _d234139