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001			234142
003			ES-VaUE
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007			cr nn 008mamaa
008			180514s2018 xxu| s |||| 0|eng d
020			_a9781493978199
024	7		_a10.1007/978-1-4939-7819-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Metabolomics _bMethods and Protocols _cedited by Carla António.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XIV, 355 páginas) _b78 ilustraciones, 51 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1778
505	0		_aThe Importance of Experimental Design, Quality Assurance, and Control in Plant Metabolomics Experiments -- Standard Key Steps in Mass Spectrometry-Based Plant Metabolomics Experiments: Instrument Performance and Analytical Method Validation -- Guidelines for Sample Normalization to Minimize Batch Variation for Large-Scale Metabolic Profiling of Plant Natural Genetic Variance -- Gas Chromatography-Mass Spectrometry-Based 13C-Labeling Studies in Plant Metabolomics -- Carbon Atomic Survey for Identification of Selected Metabolic Fluxes -- Quantification of Low-Abundant Phosphorylated Carbohydrates Using HILIC-QqQ-MS/MS -- Quantification of Soluble Sugars and Sugar Alcohols by LC-MS/MS -- Untargeted Analysis of Semi-Polar Compounds by LC-MS and Targeted Analysis of Fatty Acids by GC-MS/GC-FID: From Plant Cultivation to Extract Preparation -- Extraction of Plant Lipids for LC-MS-Based Untargeted Plant Lipidomics -- Semi-Targeted Lipidomics of Plant Acyl Lipids Using UPLC-HR-MS in Combination with a Data-Independent Acquisition Mode -- Plant Lipidomics Using UPLC-QTOF-MS -- Targeted LC-MS Analysis for Plant Secondary Metabolites -- An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones -- A Complete Pipeline for Generating a High-Resolution LC-MS-Based Reference Mass Spectra Library -- Quantification of Sugars and Organic Acids in Biological Matrices Using GC-QqQ-MS -- Acquisition of Volatile Compounds by Gas Chromatography-Mass Spectrometry (GC-MS) -- Mass Spectrometry Imaging (MSI) for Plant Metabolomics -- Laser Ablation Electrospray Ionization-Mass Spectrometry Imaging (LAESI-MS) for Spatially Resolved Plant Metabolomics -- Live Single-Cell Mass Spectrometry (LSC-MS) for Plant Metabolomics -- Statistical and Multivariate Analysis of MS-Based Plant Metabolomics Data -- Integration of Plant Metabolomics Data into Metabolic Networks: Progresses and Challenges -- Understanding the Functionality of a Biological System as a Whole: Comparative Data Analysis -- Regression-Based Modeling of Complex Plant Traits Based on Metabolomics Data -- Mathematical Modeling Approaches in Plant Metabolomics.
520			_aThis detailed book aims to address the need for standardizing key steps in plant metabolomics research that is still considered a critical issue in the field. The collection unites diverse mass spectrometry (MS)-based protocols, from sample preparation and extraction of plant primary and secondary metabolites (e.g. a range of sugars, lipids, phytohormones and volatile organic compounds) to metabolite analysis, using exclusively highly sensitive MS-based methodologies, followed by frontline bioinformatics and/or mathematical modeling approaches to produce reliable biological data interpretation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Metabolomics: Methods and Protocols serves both new and well-established researchers in the plant metabolomics field in finding helpful guidelines and useful laboratory-based protocols for setting up their routine plant metabolomics experiments.
700	1		_aAntónio, Carla _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493978182
776	0	8	_iPrinted edition: _z9781493978205
776	0	8	_iPrinted edition: _z9781493992942
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7819-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c234142 _d234142