000			03888nam a22003615i 4500
001			234143
003			ES-VaUE
005			20221220020543.0
007			cr nn 008mamaa
008			180629s2018 xxu| s |||| 0|eng d
020			_a9781493986002
024	7		_a10.1007/978-1-4939-8600-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aEpithelial Cell Culture _bMethods and Protocols _cedited by Mario Baratta.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XIII, 214 páginas) _b54 ilustraciones, 36 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1817
505	0		_aNormal Human Thyrocytes in Culture -- Isolation and Culture of Juvenile Pig Thyroid Follicular Epithelia -- Reassembly of Functional Human Salivary Stem/Progenitor Cells in 3D Culture -- Culture and Differentiation of Lung Bronchiolar Epithelial Cells In Vitro -- Differentiation of gastrointestinal cell lines by culture in semi-wet interface -- Three-dimensional cell culture model utilization in renal carcinoma cancer stem cell research -- Amniotic epithelial cell culture. Corresponding author -- Bovine granulosa cell culture -- Bioencapsulation of oocytes and granulosa cells -- Ovine granulosa cells isolation and culture to improve oocyte quality -- 3D model replicating the intestinal function to evaluate drug permeability -- Isolation of Human Gastric Epithelial Cells from Gastric Surgical Tissue and Gastric Biopsies for Primary Culture -- Long-term culture of intestinal organoids -- Bovine mammary organoids: a model to study epithelial mammary cells -- Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from organoids -- Mouse-Derived Gastric Organoid and Immune Cell Co-Culture for the Study of the Tumor Microenvironment -- Murine and human mammary cancer cell lines: functional tests -- In vitro porcine colon culture -- Primary cultures of olfactory neurons from the avian olfactory epithelium.
520			_aThis detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells from different organs, gastrointestinal system, thyroid, salivary gland, ovary, mammary gland, and olfactory epithelial tissue. Cell culture is a fundamental technique in both medical research and drug discovery and two-dimensional (2D) culture has been the preferred method, due to the ease with which cell monolayers can be induced to proliferate on planar surfaces. The book propose several functional assay useful to test cell activities. Further, The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures. Today, 3D cell cultures are emerging not only as a new tool in early drug discovery, but also as potential therapeutics to treat disease. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab.
700	1		_aBaratta, Mario _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493985999
776	0	8	_iPrinted edition: _z9781493986019
776	0	8	_iPrinted edition: _z9781493993390
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8600-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c234143 _d234143