000			03441nam a22003735i 4500
001			234144
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007			cr nn 008mamaa
008			180630s2018 xxu| s |||| 0|eng d
020			_a9781493986033
024	7		_a10.1007/978-1-4939-8603-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMouse Oocyte Development _bMethods and Protocols _cedited by Marie-Hélène Verlhac, Marie-Emilie Terret.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 174 páginas) _b43 ilustraciones, 30 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1818
505	0		_aGrowth In Vitro of Granulosa Cell-oocyte Complexes of the Mouse -- In vitro Culture of Mouse Oocytes for Meiotic Maturation -- In vitro Maturation and Culture of Human Oocytes -- Pronuclear Transfer in Human Oocytes -- In vitro Fertilization of Mouse Oocyte -- Profiling Maternal mRNA Translation during Oocyte Development -- Transcriptome Profiling of Single Mouse Oocytes -- Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation -- Immunofluorescence Staining of K-fibres in Mouse Oocytes Using Cold-fixation -- Triple-colour Live Imaging of Mouse Oocytes -- Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes -- Manipulating Cohesin Levels in Live Mouse Oocytes -- Optogenetic Manipulation of Mouse Oocytes -- Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin -- Photoactivation of Actin in Mouse Oocyte -- Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes -- Micropipette Aspiration of Oocytes to Assess Cortical Tension.
520			_aThis book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.
700	1		_aVerlhac, Marie-Hélène _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aTerret, Marie-Emilie _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493986026
776	0	8	_iPrinted edition: _z9781493986040
776	0	8	_iPrinted edition: _z9781493993406
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8603-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c234144 _d234144