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001			234145
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007			cr nn 008mamaa
008			180629s2018 xxu| s |||| 0|eng d
020			_a9781493986248
024	7		_a10.1007/978-1-4939-8624-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_amiRNA Biogenesis _bMethods and Protocols _cedited by Ulf Andersson Vang Ørom.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing : _bImprint: Springer, _c2018
300			_a1 recurso en línea (XI, 238 páginas) _b58 ilustraciones, 45 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1823
505	0		_aHigh-throughput characterization of primary microRNA transcripts -- Identifying pri-miRNA transcription start sites -- Metabolic pulse-chase RNA labelling for pri-miRNA processing dynamics -- In Vitro System for Coupling RNAP II Transcription to Primary microRNA Processing and a Three-way System for RNAP II Transcription/Splicing/microRNA Processing -- Purification of Microprocessor-associated factors -- Arresting pri-miRNA processing with Target Site Blockers -- MicroRNA analysis using the quantitative real-time PCR reaction -- MicroRNA analysis using next-generation sequencing -- Identification of microRNA precursor-associated proteins -- Analysis of 3' end modifications in (precursor-) microRNAs by high-throughput -- Uncovering the stability of mature miRNAs by 4-thio-uridine metabolic labeling -- Detection of microRNA-target interactions by Chimera PCR (ChimP) -- High-quality overlapping paired-end reads for the detection of A-to-I editing on small RNA -- Targeting miRNA for therapeutics using a Micronome based method for identification of miRNA-mRNA pairs and validation of key regulator miRNA -- Method for detection of miRNAs in non-model organisms with unreported database -- Detection and Verification of Mammalian Mirtrons by Northern Blotting -- Detecting agotrons in Ago CLIPseq data.
520			_aThis detailed volume collects chapters that seek to expand our knowledge of molecular events and extended molecular networks in gene regulation. The contents explore numerous aspects of miRNA biogenesis, a sophisticated series of events that assure a finely tuned regulation of miRNA expression and activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, miRNA Biogenesis: Methods and Protocols aims to contribute to the further development of knowledge and application of miRNA biogenesis, both in the basic research lab and in the clinic.
700	1		_aØrom, Ulf Andersson Vang _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493986231
776	0	8	_iPrinted edition: _z9781493986255
776	0	8	_iPrinted edition: _z9781493993451
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8624-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c234145 _d234145