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008			150304s2015 xxu| s |||| 0|eng d
020			_a9781493924530
024	7		_a10.1007/978-1-4939-2453-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Gene Silencing _bMethods and Protocols _cedited by Kirankumar S. Mysore, Muthappa Senthil-Kumar.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XI, 307 páginas) _b34 ilustraciones, 17 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1287
505	0		_aAdvanced in Plant Gene Silencing Methods -- Strategies for Altering Plant Traits Using Virus-Induced Gene Silencing Technologies -- Bioinformatics Tools for Achieving Better Gene Silencing in Plants -- Profiling of Small RNAs Involved in Plant-Pathogen Interactions -- RNAi-Mediated Resistance to Viruses in Genetically Engineered Plants -- Simplifying Transgene Locus Structure Through Cre-lox Recombination -- Transgene-Induced Gene Silencing in Plants -- Gene Silencing by DNA Interference in Fern Gametophytes -- Induction of Stable Epigenetic Gene Silencing in Plants Using a Virus Vector -- A Method for Validating MicroRNAs in Plants by miR-RACE -- MR VIGS: MicroRNA-Based Virus-Induced Gene Silencing in Plants -- A High-Throughput RNA Interference (RNAi)-Based Approach Using Hairy Roots for the Study of Plant-Rhizobia Interactions -- A Functional Genomics Method for Assaying Gene Function in Phytopathogenic Fungi Through Host-Induced Gene Silencing Mediated by Agroinfiltration -- An Effective and Convenient Method for the Delivery of Apple Latent Spherical Virus (ALSV)-Based Vectors into Plant Cells by Agroinoculation -- Virus-Induced Gene Silencing (VIGS) for Functional Genomics in Rice Using rice tungro bacilliform virus (RTBV) as a Vector -- Virus-Induced Gene Silencing of Fiber-Related Genes in Cotton -- Establishment of an Efficient Virus-Induced Gene Silencing (VIGS) Assay in Arabidopsis by Agrobacterium-Mediated Rubbing Infection -- Virus-Induced Gene Silencing as a Scalable Tool to Study Drought Tolerance in Plants -- VIGS for Dissecting Mechanisms Involved in the Symbiotic Interaction of Microbes with Plants -- Construction of a Cotton VIGS Library for Functional Genomic Study -- Synthetic Gene Complementation to Determine Off-Target Silencing -- Construction of Mismatched Inverted Repeat (IR) Silencing Vectors for Maximizing IR Stability and Effective Gene Silencing and in Plants.
520			_aThis volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.
700	1		_aMysore, Kirankumar S _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSenthil-Kumar, Muthappa _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493924547
776	0	8	_iPrinted edition: _z9781493924523
776	0	8	_iPrinted edition: _z9781493948475
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2453-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234146 _d234146