000			03189nam a22003615i 4500
001			234148
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007			cr nn 008mamaa
008			141112s2015 xxu| s |||| 0|eng d
020			_a9781493920808
024	7		_a10.1007/978-1-4939-2080-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aAdvanced Fluorescence Microscopy _bMethods and Protocols _cedited by Peter J. Verveer.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XI, 294 páginas) _b86 ilustraciones, 65 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1251
505	0		_aThe Physical Basis Of Total Internal Reflection Fluorescence (TIRF) Microscopy and Its Cellular Applications -- Two-Photon Excitation Microscopy And Its Applications In Neuroscience -- Live Spheroid Formation Recorded with Light Sheet-based Fluorescence Microscopy -- Fluorescence Microscopy Based RNA Interference Screening -- Fluorescence Resonance Energy Transfer Microscopy (FRET) -- Localizing Protein-Protein Interactions In Living Cells Using Fluorescence Lifetime Imaging Microscopy -- Analysis Of Biomolecular Dynamics By FRAP And Computer Simulation -- Fluorescence Correlation Spectroscopy -- Homo-FRET Imaging Highlights The Nanoscale Organization Of Cell Surface Molecules -- Practical Structured-Illumination Microscopy -- 4Pi Microscopy of the Nuclear Pore Complex -- Application Of STED Microscopy To Cell Biology Questions -- Three-Dimensional Photoactivated Localization Microscopy With Genetically-Expressed Probes -- Direct Stochastic Optical Reconstruction Microscopy (dSTORM) -- Optogenetics: Optical Control Of A Photoactivatable Rac In Living Cells.
520			_aThis volume provides an overview of advanced  fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems.  Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge,  Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.
700	1		_aVerveer, Peter J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493920815
776	0	8	_iPrinted edition: _z9781493920792
776	0	8	_iPrinted edition: _z9781493942527
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2080-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234148 _d234148