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020 _a9781493972920
024 7 _a10.1007/978-1-4939-7292-0
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlant Respiration and Internal Oxygen
_bMethods and Protocols
_cedited by Kapuganti Jagadis Gupta.
250 _a1st edition 2017
264 1 _aNew York, NY
_bSpringer International Publishing
_c2017
300 _a1 recurso en línea (XIII, 285 páginas)
_b74 ilustraciones, 58 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1670
505 0 _aAssessing Metabolic Flux in Plants with Radiorespirometry -- Coupling Radiotracer Experiments with Chemical Fractionation for the Estimation of Respiratory Fluxes -- A method for Imaging Oxygen Distribution and Respiration at a Microscopic Level of Resolution -- VisiSens Technique to Measure Internal Oxygen and Respiration in Barley Roots -- MultiSense: A Multimodal Sensor Tool Enabling the High Throughput Analysis of Respiration -- Measurement of Respiration and Internal Oxygen in Germinating Cicer arietinum L. Seeds Using Micro Sensor -- Using an Oxygen Microsensor to Measure Oxygen Dynamics in Tomato Plants in Response to Pseudomonas syringe Infection -- Measurement of Oxygen Status in Arabidopsis Leaves Undergoing Hypersensitive Response During Pseudomonas Infection -- Isolation of Physiologically Active and Intact Mitochondria from Chickpea -- Isolation and Structural Studies of Mitochondria from Pea Roots -- Mitochondrial Respiration and Oxygen Tension -- Isolation of Mitochondria from Model and Crop Plants -- Procedures of Mitochondria Purification and Gene Expression to Study Alternative Respiratory and Uncoupling Pathways in Fruits -- Measurement of Tricarboxylic Acid Cycle Enzyme Activities in Plants -- Respiration Traits as Novel Markers for Plant Robustness under the Threat of Climate -- Calorespirometry - A Novel Tool in Functional Hologenomics to Select 'Green' Holobionts for Biomass Production -- Measurements of Electron Partitioning Between Cytochrome and Alternative Oxidase Pathways in Plant Tissues -- A Driving Bioinformatics Approach to Explore Co-regulation of AOX Gene Family Members during Growth and Development -- A Step-by-Step Protocol for Classifying AOX Proteins in Flowering Plants -- Studying Individual Plant AOX Gene Functionality in Early Growth Regulation - A New Approach -- Laser-Capture Microdissection for Amplification of Alternative Oxidase (AOX) Genes in Target Tissues in Daucus carota L. -- Measurement of Mitochondrial Respiration in Isolated Protoplasts: Cytochrome and Alternative Pathways -- Measuring Spatial and Temporal Oxygen Flux near Plant Tissues using a Self-Referencing Optrode. .
520 _aThis volume covers a wide range of methods to measure cellular respiration and internal oxygen in various tissues under different conditions. Chapters guide readers through informative experimental approaches, calorespirometry, isotope fractionation techniques, protocols for dual-inlet isotope ratio mass spectrometry, laser-capture microdissection, and bioinformatics approach for exploring the co-regulation of AOX gene family members. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Respiration and Internal Oxygen: Methods and Protocols aims to be helpful for all students and researchers interested in the determination of respiration and internal oxygen. .
700 1 _aJagadis Gupta, Kapuganti
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493972913
776 0 8 _iPrinted edition:
_z9781493972937
776 0 8 _iPrinted edition:
_z9781493984442
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7292-0
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2017
999 _c234162
_d234162