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008			170811s2017 xxu| s |||| 0|eng d
020			_a9781493972234
024	7		_a10.1007/978-1-4939-7223-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMammalian Synthetic Promoters _cedited by David Gould.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 277 páginas) _b43 ilustraciones, 25 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1651
505	0		_aInitial Considerations before Designing a Promoter Construct -- Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay -- Chromatin Immunoprecipitation and Quantitative Real-time PCR to Assess Binding of a Protein of Interest to Identified Predicted Binding Sites within a Promoter -- Secreted Reporters for Monitoring Multiple Promoter Function -- Bioluminescence Monitoring Of Promoter Activity In Vitro and In Vivo -- Monitoring Promoter Activity by Flow Cytometry -- Functional Screening of Core Promoter Activity -- Bioinformatically Informed Design of Synthetic Mammalian Promoters -- Synthetic Tumor Specific Promoters for Transcriptional Regulation of Viral Replication -- Constructing Strong Cell-Type-Specific Promoters through Informed Design -- PCR Assembly of Synthetic Promoters -- The Tetracycline Responsive System -- Light-responsive Promoters -- A Simple Method for Constructing Artificial Promoters Activated in Response to Ultrasound Stimulation -- Promoter Activation with Electromagnetism -- Application of Synthetic Tumor Specific Promoters Responsive to the Tumor Microenvironment -- A Modular Approach to Building Complex Synthetic Circuits -- Computational Sequence Design with R2oDNA Designer -- Design of Synthetic Promoters for Gene Circuits in Mammalian Cells. .
520			_aThis volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammalian Synthetic Promoters: Methods and Protocols aims to aid researchers that are new to the field of synthetic promoters and inspire new developments.
700	1		_aGould, David _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493972210
776	0	8	_iPrinted edition: _z9781493972227
776	0	8	_iPrinted edition: _z9781493984213
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7223-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234164 _d234164