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008			160603s2016 xxu| s |||| 0|eng d
020			_a9781493936434
024	7		_a10.1007/978-1-4939-3643-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aType-1 Diabetes _bMethods and Protocols _cedited by Kathleen M. Gillespie.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (X, 213 páginas) _b44 ilustraciones, 33 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1433
505	0		_aType 1 Diabetes: Prediction And Prevention: Current Perspectives -- The HLA -- Pancreatic Beta Cell Survival and Signaling Pathways: Effects of Type 1 Diabetes-Associated Genetic Variants -- Islet Autoantibody Analysis: Radioimmunoassays -- Islet Autoantibody Analysis: Non-Radioactive Assays - Electrochemiluminescence -- Detection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function in Type 1 Diabetes -- Histology of the Type 1 Diabetes Pancreas -- Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes -- Tracking Immunological Responses of Islet Antigen-Specific T Cells In The Non Obese Diabetic (NOD) Mouse Model Of Type 1 Diabetes -- Adoptive Transfer of Autoimmune Diabetes using Immunodeficient Non Obese Diabetic (NOD) Mice -- Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes -- FISH with Concomitant Immunofluorescence -- Laser Capture Microdissection: Single Pancreatic Cell DNA Profiling -- The Gut Microbiome in the NOD Mouse -- Molecular Methods and Protein Synthesis for Definition of Autoantibody Epitopes. .
520			_aThe aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and reliable, Type-1 Diabetes: Methods and Protocols will aid researchers in using these methods to advance their own studies.
700	1		_aGillespie, Kathleen M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493936410
776	0	8	_iPrinted edition: _z9781493936427
776	0	8	_iPrinted edition: _z9781493981045
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3643-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234166 _d234166