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008			150209s2015 xxu| s |||| 0|eng d
020			_a9781493923922
024	7		_a10.1007/978-1-4939-2392-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aBacterial Transcriptional Control _bMethods and Protocols _cedited by Irina Artsimovitch, Thomas J. Santangelo.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XI, 342 páginas) _b48 ilustraciones, 25 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1276
505	0		_aMapping the Escherichia coli Transcription Elongation Complex with Exonuclease III -- Purification of Bacterial RNA Polymerase: Tools and Protocols -- Monitoring Translocation of Multisubunit RNA Polymerase Along the DNA with Fluorescent Base Analogues -- In vitro and in vivo Methodologies for Studying the Sigma54-dependent Transcription -- Methods for the Assembly and Analysis of in vitro Transcription-coupled-to-translation Systems -- Site-specific Incorporation of Probes into RNA Polymerase by Unnatural-amino-acid Mutagenesis and Staudinger-Bertozzi Ligation -- Reconstitution of Factor-dependent, Promoter Proximal Pausing in Drosophila Nuclear Extracts -- Direct Competition Assay for Transcription Fidelity -- Single-stranded DNA Aptamers for Functional Probing of Bacterial RNA Polymerase -- Biochemical Analysis of Transcription Termination by RNA Polymerase III from Yeast Saccharomyces cerevisiae -- Use of RNA Polymerase Molecular Beacon Assay to Measure RNA Polymerase Interactions with Model Promoter Fragments -- Preparation of cDNA Libraries for High-throughput RNA Sequencing Analysis of RNA 5' Ends -- In situ Footprinting of E. coli Transcription Elongation Complex with Chloroacetaldehyde -- Using Solutes and Kinetics to Probe Large Conformational Changes in the Steps of Transcription Initiation -- Manipulating Archaeal Systems to Permit Analyses of Transcription Elongation-termination Decisions in vitro -- Purification of Active RNA Polymerase I from Yeast -- Transcription in Archaea: preparation of Methanocaldococcus jannaschii Transcription Machinery -- Transcription in Archaea: in vitro Transcription Assays for mjRNAP -- Experimental Analysis of hFACT Action during Pol II Transcription in vitro -- ChIP-seq for Genome-scale Analysis of Bacterial DNA-binding Proteins.
520			_aThis volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of in vitro and in vivo technologies that will permit researchers to purify and probe the position and stability of RNA polymerase complexes at different points of the transcription cycle, analyze the various translocations and intermolecular movements associated with catalysis, define recruitment strategies, probe the roles of transcription factors in each stage of the cycle, highlight conserved and disparate fidelity mechanisms, analyze the resultant transcripts, and study coordination of the nascent mRNA synthesis by the RNA polymerase and mRNA translation by the ribosome. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls. Practical and timely, Bacterial Transcriptional Controls: Methods and Protocols highlights the breadth and depth of techniques that are likely to continue shaping the transcription community in the future.
700	1		_aArtsimovitch, Irina _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSantangelo, Thomas J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493923939
776	0	8	_iPrinted edition: _z9781493923915
776	0	8	_iPrinted edition: _z9781493954674
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2392-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234172 _d234172