000			03347nam a22003495i 4500
001			234175
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007			cr nn 008mamaa
008			100301s2007 xxu| s |||| 0|eng d
020			_a9781597452090
024	7		_a10.1007/978-1-59745-209-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMacromolecular Crystallography Protocols, Volume 1 _bPreparation and Crystallization of Macromolecules _cedited by Sylvie Doublie.
250			_a1st edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XIV, 280 páginas) _b49 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v363
505	0		_aA Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag -- Cloning, Production, and Purification of Proteins for a Medium-Scale Structural Genomics Project -- Baculoviral Expression of an Integral Membrane Protein for Structural Studies -- Protein Engineering -- Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems -- How to Use Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals -- Screening and Optimization Methods for Nonautomated Crystallization Laboratories -- Improving Marginal Crystals -- Optimization Techniques for Automation and High Throughput -- Three-Dimensional Crystallization of Membrane Proteins -- Crystallization of Protein-DNA Complexes -- Preparation and Crystallization of RNA -- Crystallization of RNA-Protein Complexes.
520			_aMacromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators. Volume 1 is composed of detailed protocols for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization, as well as computational techniques for data collection, phasing, and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure.
700	1		_aDoublie, Sylvie _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617374753
776	0	8	_iPrinted edition: _z9781588292926
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-209-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2007
999			_c234175 _d234175