000			03739nam a22003855i 4500
001			234176
003			ES-VaUE
005			20221220020546.0
007			cr nn 008mamaa
008			161118s2017 gw | s |||| 0|eng d
020			_a9783662504284
024	7		_a10.1007/978-3-662-50428-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHydrocarbon and Lipid Microbiology Protocols _bPrimers _cedited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales.
250			_a1st edition 2017
264		1	_aBerlin, Heidelberg _bSpringer International Publising _c2017
300			_a1 recurso en línea (X, 241 páginas) _b10 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aIntroduction -- Current landscape of biomolecular approaches for assessing biodegradation of aromatic hydrocarbons -- Primers - bacterial genes encoding enzymes for aerobic alkane degradation -- Primers - functional genes for anaerobic hydrocarbon degrading microbes -- Primers - functional marker genes for methylotrophs and methanotrophs -- Primers - functional genes and 16S rRNA genes for methanogens -- Primers - functional genes for aerobic chlorinated hydrocarbon degrading microbes -- Primers that target functional genes of organohalide-respiring bacteria -- Primers - functional genes for nitrogen-cycling microbes in oil reservoirs.
520			_aThis Volume addresses the pros and cons of oligonucleotide probes, primers and primer combinations, and importantly considers how to design the best tools for the microbial taxa and/or processes being investigated. Individual chapters focus on the design of primers targeting genes that code for enzymes associated with the following functions: degradation of aromatic, aliphatic and chlorinated hydrocarbons under aerobic and anaerobic conditions, methanogenesis, methane oxidation, and the nitrogen cycle. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
700	1		_aMcGenity, Terry J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aTimmis, Kenneth N _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aNogales, Balbina _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9783662504277
776	0	8	_iPrinted edition: _z9783662504291
776	0	8	_iPrinted edition: _z9783662570579
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-662-50428-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234176 _d234176