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008			170503s2017 xxu| s |||| 0|eng d
020			_a9781493968879
024	7		_a10.1007/978-1-4939-6887-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHeterologous Gene Expression in E.coli _bMethods and Protocols _cedited by Nicola A. Burgess-Brown.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XIV, 429 páginas) _b92 ilustraciones, 55 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1586
505	0		_aRecombinant Protein Expression in E. coli: A Historical Perspective -- N- and C-Terminal Truncations to Enhance Protein Solubility and Crystallization: Predicting Protein Domain Boundaries with Bioinformatics Tools -- Harnessing the Profinity eXactTM System for Expression and Purification of Heterologous Proteins in E. coli -- ESPRIT: A Method for Defining Soluble Expression Constructs in Poorly Understood Gene Sequences -- Optimizing Expression and Solubility of Proteins in E. coli Using Modified Media and Induction Parameters -- Optimization of Membrane Protein Production Using Titratable Strains of E. coli -- Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) or pReX and GFP-Fusions -- High Yield of Recombinant Protein in Shaken E. coli Cultures with Enzymatic Glucose Release Medium EnPresso B -- A Generic Protocol for Purifying Disulphide-Bonded Domains and Random Protein Fragments Using Fusion Proteins with SUMO3 and Cleavage by SenP2 Protease -- A Strategy for Production of Correctly Folded Disulfide-Rich Peptides in the Periplasm of E. coli -- Split GFP Complementation as Reporter of Membrane Protein Expression and Stability in E. coli: A Tool to Engineer Stability in a LAT Transporter -- Acting on Folding Effectors to Improve Recombinant Protein Yields and Functional Quality -- Protein Folding Using a Vortex Fluidic Device -- Removal of Affinity Tags with TEV Protease -- Generation of Recombinant N-Linked Glycoproteins in E. coli -- Production of Protein Kinases in E. coli -- Expression of Prokaryotic Integral Membrane Proteins in E. coli -- Multiprotein Complex Production in E. coli: The SecYEG-SecDFYajC-YidC Holotranslocon -- Membrane Protein Production in E. coli Lysates in Presence of Pre-Assembled Nanodiscs -- Not Limited to E. coli: Versatile Expression Vectors for Mammalian Protein Expression -- A Generic Protocol for Intracellular Expression of Recombinant Proteins in Bacillus subtilis -- In Vivo Biotinylation of Antigens in E. coli -- Cold-Shock Expression System in E. coli for Protein NMR Studies -- High-Throughput Production of Proteins in E. coli for Structural Studies -- Mass Spectrometric Analysis of Proteins -- How to Determine Interdependencies of Glucose and Lactose Uptake Rates for Heterologous Protein Production with E. coli -- Interfacing Biocompatible Reactions with Engineered Escherichia coli.
520			_aThis detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism.
700	1		_aBurgess-Brown, Nicola A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493968855
776	0	8	_iPrinted edition: _z9781493968862
776	0	8	_iPrinted edition: _z9781493983285
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6887-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234183 _d234183