000			04224nam a22003735i 4500
001			234184
003			ES-VaUE
005			20221220020546.0
007			cr nn 008mamaa
008			100301s2009 xxu| s |||| 0|eng d
020			_a9781597454711
024	7		_a10.1007/978-1-59745-471-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aGene Knockout Protocols _cedited by Ralf Kühn, Wolfgang Wurst.
250			_a2nd edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XVI, 516 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v530
505	0		_aGene Modification in ES Cells -- Overview on Mouse Mutagenesis -- Construction of Gene-Targeting Vectors by Recombineering -- Gene-Trap Vectors and Mutagenesis -- Chromosome Engineering in ES Cells -- Gene Modification in Embryonic Stem Cells by Single-Stranded DNA Oligonucleotides -- Generation of shRNA Transgenic Mice -- Mutagenesis of Mouse Embryonic Stem Cells with Ethylmethanesulfonate -- Stem Cell Manipulation -- Gene Targeting in Mouse Embryonic Stem Cells -- Manipulating Mouse Embryonic Stem Cells -- ES Cell Line Establishment -- Generation of Double-Knockout Embryonic Stem Cells -- Differentiation Analysis of Pluripotent Mouse Embryonic Stem (ES) Cells In Vitro -- Cloning of ES Cells and Mice by Nuclear Transfer -- Genetically Engineered Mice -- Isolation, Microinjection and Transfer of Mouse Blastocysts -- Aggregation Chimeras: Combining ES Cells, Diploid, and Tetraploid Embryos -- VelociMouse: Fully ES Cell-Derived F0-Generation Mice Obtained from the Injection of ES Cells into Eight-Cell-Stage Embryos -- Generation of Cre Recombinase-Expressing Transgenic Mice Using Bacterial Artificial Chromosomes -- Inducible Cre Mice -- Creation and Use of a Cre Recombinase Transgenic Database -- Transposon Mutagenesis in Mice -- Lentiviral Transgenesis -- Sperm Cryopreservation and In Vitro Fertilization -- Phenotype Analysis -- Influence of Genetic Background on Genetically Engineered Mouse Phenotypes -- Pathologic Phenotyping of Mutant Mice -- Systemic First-Line Phenotyping.
520			_aWith the completion of the mouse and human genome sequences, the functional characterization of every mammalian gene and the deciphering of their molecular interaction network has become the next major challenge, and the mouse genome provides a convenient and vital model for these studies. In Gene Knockout Protocols, Second Edition, distinguished contributors with extensive experience in the gene targeting and mouse genetics fields reveal a comprehensive collection of step-by-step laboratory protocols. Emphasizing the many new mutagenesis techniques developed over the last seven years, the volume covers topics such as gene modification in ES cells, stem cell manipulation, the generation of genetically engineered mice, and mutant phenotype analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Gene Knockout Protocols, Second Edition provides a unique collection of expert bench protocols certain to be a valuable resource for those just entering the field of gene targeting but also for all genetic researchers today.
700	1		_aKühn, Ralf _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWurst, Wolfgang _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607613060
776	0	8	_iPrinted edition: _z9781934115268
776	0	8	_iPrinted edition: _z9781627038348
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-471-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c234184 _d234184