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020 _a9781493935970
024 7 _a10.1007/978-1-4939-3597-0
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aForensic DNA Typing Protocols
_cedited by William Goodwin.
250 _a2nd edition 2016
264 1 _aNew York, NY
_bSpringer International Publishing
_c2016
300 _a1 recurso en línea (X, 298 páginas)
_b28 ilustraciones, 18 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1420
505 0 _aCollection of Samples for DNA Analysis -- Body Fluid Identification Using mRNA Profiling -- mRNA Profiling for Vaginal Fluid and Menstrual Blood Identification -- Preservation of and DNA Extraction from Muscle Tissue -- DNA Extraction: Organic and Solid-Phase -- Extraction of DNA from Skeletal Remains -- Extraction of DNA from Human Skeletal Material -- The Development and Use of Internal Amplification Controls (IACs) with DNA Profiling Kits for Forensic DNA Analysis -- NucleoSpin ®XS Columns for DNA Concentration and Clean-Up -- Purification of PCR Products to Improve STR Profiles -- Analysis of 30 Biallelic INDEL Markers Using the Investigator DIPplex ® Kit -- Analysis of Mitochondrial Control Region Using Sanger Sequencing -- Whole Human Mitochondrial DNA Sequencing -- In-Solution Hybridization for the Targeted Enrichment of the Whole Mitochondrial Genome -- Enhanced DNA Profiling of the Semen Donor in Late Reported Sexual Assaults: Use of Y-Chromosome Targeted Pre-Amplification and Next Generation Y-STR Amplification Systems -- Analysis of Rapidly Mutating Y Chromosome Short Tandem Repeats (RM Y STRs) -- A Practical Guide to the HIrisPlex System: Simultaneous Prediction of Eye and Hair Color from DNA -- Inference of Ancestry in Forensic Analysis I: Autosomal Ancestry - Informative Marker Sets -- Inference of Ancestry in Forensic Analysis II: Analysis of Genetic Data -- Species Determination: The Role and Use of the Cytochrome b Gene.
520 _aThis volume presents a series of protocols and methods, some of which are not widely used by researchers/practitioners, and will aid in the execution of different laboratory techniques. Forensic DNA Typing Protocols, Second Edition is arranged into a series of related chapters. Chapter 1-3 examines two different aspects of RNA analysis for body fluid identification. Chapters 4-7 focuses on the storage of biological materials and the extraction of DNA from hard tissues. Chapters 8-10 present methods for monitoring the quality of DNA extracts, and steps to aid in the purification of DNA. Chapters 11-16 talk about methods on non-standard markers, such as INDELs, Y chromosome STRs, and mitochondrial DNA. Detailed procedures and data analysis for phenotypes and ancestry are explored in Chapter 17-19. The last chapter (20) looks at the application of DNA typing to the identification of non-human material to species level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, Forensic DNA Typing Protocols, Second Edition, is a valuable resource for forensic specialists, researchers, and anyone interested in the field of forensic science. .
700 1 _aGoodwin, William
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493935956
776 0 8 _iPrinted edition:
_z9781493935963
776 0 8 _iPrinted edition:
_z9781493980895
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3597-0
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2016
999 _c234185
_d234185