000 04341nam a22003615i 4500
001 234196
003 ES-VaUE
005 20221220020547.0
007 cr nn 008mamaa
008 161021s2017 xxu| s |||| 0|eng d
020 _a9781493964994
024 7 _a10.1007/978-1-4939-6499-4
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aEnzyme Stabilization and Immobilization
_bMethods and Protocols
_cedited by Shelley D. Minteer.
250 _a2nd edition 2017
264 1 _aNew York, NY
_bSpringer International Publishing
_c2017
300 _a1 recurso en línea (X, 234 páginas)
_b52 ilustraciones, 24 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1504
505 0 _aIntroduction to the Field of Enzyme Immobilization and Stabilization -- Stabilization of Enzymes through Encapsulation in Liposomes -- Micellar Enzymology for Thermal, pH, and Solvent Stability -- Lipase Activation and Stabilization in Room Temperature Ionic Liquids -- Nanoporous Gold for Enzyme Immobilization -- Enzyme Stabilization via Bio-Templated Silicification Reactions -- Covalent Immobilization of Enzymes on Eupergit® Supports: Effect of the Immobilization Protocol -- Micellar Polymer Encapsulation of Enzymes -- Cross-Linked Enzyme Aggregates for Applications in Aqueous and Non-Aqueous Media -- Protein Coated Microcrystals, Combi-Protein Coated Microcrystals and Cross-Linked Protein Coated Microcrystals of Enzymes for Use in Low Water Media -- Macroporous Poly(GMA-co-EGMA) for Enzyme Stabilization -- Cytochrome C Stabilization and Immobilization in Aerogels -- Enzyme Immobilization and Mediation with Osmium Redox Polymers -- Ferrocene-Modified Linear Poly(ethylenimine) for Enzymatic Immobilization and Electrom Mediation -- Fad-Dependent Glucose Dehydrogenase Immobilization and Mediation within a Naphthoquinone Redox Polymer -- Layer-by-Layer Assembly of Glucose Oxidase on Carbon Nanotube Modified Electrodes -- Kinetic Measurement for Enzyme Immobilization.
520 _aThis volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including liposome formation, micelle introduction, crosslinking, and additives. The second part of the book discusses protocols for enzyme stabilization during enzyme immobilization, including common techniques like sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Protocols for a variety of enzymes are shown, but the enzymes are chosen as examples to show that these protocols can be used for both enzymes of biological importance, as well as enzymes of industrial importance. The final part details spectroscopic protocols, methods, and assays for studying the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Enzyme Stabilization and Immobilization: Methods and Protocols, Second Edition provides molecular biologists, biochemists, and biomedical and biochemical engineers with the state-of-the-art technical information required to effectively stabilize their enzyme of interest in a variety of environments (i.e., harsh temperature, pH, or solvent conditions).
700 1 _aMinteer, Shelley D
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493964970
776 0 8 _iPrinted edition:
_z9781493964987
776 0 8 _iPrinted edition:
_z9781493982196
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6499-4
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2017
999 _c234196
_d234196