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001			234199
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007			cr nn 008mamaa
008			110805s2011 xxu| s |||| 0|eng d
020			_a9781617792342
024	7		_a10.1007/978-1-61779-234-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aChloroplast Research in Arabidopsis _bMethods and Protocols, Volume I _cedited by R. Paul Jarvis.
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (X, 374 páginas) _b60 ilustraciones, 30 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v774
505	0		_aScreening or Selection for Chloroplast Biogenesis Mutants of Arabidopsis, Following Chemical or Insertional Mutagenesis -- Analysis of Plastid Number, Size, and Distribution in Arabidopsis Plants by Light and Fluorescence Microscopy -- Immunofluorescence Microscopy for Localization of Arabidopsis Chloroplast Proteins -- Transient Expression and Analysis of Chloroplast Proteins in Arabidopsis Protoplasts -- Visualisation of Stromules on Arabidopsis Plastids -- Analysis of Chloroplast Movement and Relocation in Arabidopsis -- Studying Starch Content and Sedimentation of Amyloplast Statoliths In Arabidopsis Roots -- Studying Arabidopsis Chloroplast Structural Organization Using Transmission Electron Microscopy -- Transplastomics in Arabidopsis: Progress Towards Developing an Efficient Method -- Isolation, Quantification, and Analysis of Chloroplast DNA -- Measurement of Transcription Rates in Arabidopsis Chloroplasts -- Studying the Structure and Processing of Chloroplast Transcripts -- In Vitro RNA Binding Assay for Studying Trans-Factors for RNA Editing in Chloroplasts -- Studying Translation in Arabidopsis Chloroplasts -- Studying Proteases and Protein Turnover in Arabidopsis Chloroplasts -- In Silico Methods for the Identification of Organellar and Suborganellar Targeting Peptides in Arabidopsis Chloroplast Proteins and for Predicting the Topology of Membrane Proteins -- Rapid Isolation of Arabidopsis Chloroplasts and Their Use for In Vitro Protein Import Assays -- Energetic Manipulation of Chloroplast Protein Import and the Use of Chemical Cross-Linkers to Map Protein-Protein Interactions -- Isolation of Arabidopsis Thylakoid Membranes and Their Use for In Vitro Protein Insertion or Transport Assays -- Determining the Location of an Arabidopsis Chloroplast Protein Using In Vitro Import Followed by Fractionation and Alkaline Extraction -- Studying Arabidopsis Envelope Protein Localization and Topology Using Thermolysin and Trypsin Proteases.
520			_aChloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable.  The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid.  In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume I explores topics such as genetics, cytology, in vivo analysis, gene expression, and protein accumulation, as well as protein transport, localization, and topology.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.
700	1		_aJarvis, R. Paul _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617792335
776	0	8	_iPrinted edition: _z9781617792359
776	0	8	_iPrinted edition: _z9781493958504
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-234-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2011
999			_c234199 _d234199