000			03779nam a22003615i 4500
001			234208
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007			cr nn 008mamaa
008			170922s2018 xxu| s |||| 0|eng d
020			_a9781493972715
024	7		_a10.1007/978-1-4939-7271-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSingle Molecule Analysis _bMethods and Protocols _cedited by Erwin J. G. Peterman.
250			_a2nd edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 369 páginas) _b88 ilustraciones, 46 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1665
505	0		_aIntroduction to Optical Tweezers: Background, System Designs, and Commercial Solutions -- RNA Unzipping and Force Measurements with a Dual Optical Trap -- Protein Tethering for Folding Studies -- Combining Structure-Function and Single-Molecule Studies on Cytoplasmic Dynein -- A Brief Introduction to Single-Molecule Fluorescence Methods -- Fluorescent Labeling of Proteins -- Single-Molecule Imaging of Escherichia coli Trans-Membrane Proteins -- Single-Molecule Fluorescence Microscopy in Living Caenorhabditis elegans -- Purification and Application of a Small Actin Probe for Single-Molecule Localization Microscopy -- Fluorescence Microscopy of Nanochannel-Confined DNA -- Use of Single Molecule Fluorescence Polarization Microscopy to Study Protein Conformation and Dynamics of Kinesin--Microtubule Complexes -- Single Molecule FRET Analysis of DNA Binding Proteins -- Atomic Force Microscopy: An Introduction -- Imaging of DNA and Protein by SFM and Combined SFM-TIRF Microscopy -- Atomic Force Microscopy of Protein Shells: Virus Capsids and Beyond -- Combined Magnetic Tweezers and Micro-Mirror Total Internal Reflection Fluorescence Microscope for Single Molecule Manipulation and Visualization -- Tethered Particle Motion: An Easy Technique for Probing DNA Topology and Transcription Factor Interactions -- Single-Molecule Measurements Using Acoustic Force Spectroscopy (AFS) -- Repurposing a Benchtop Centrifuge for High-Throughput Single-Molecule Force Spectroscopy. .
520			_aThe aim of this volume is to provide a broad overview of single-molecule approaches applied to biomolecules. Chapters in Single Molecule Techniques: Methods and Protocols, Second Edition detail the most widely used single-molecule techniques, such as DNA, DNA-binding proteins, motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Single Molecule Analysis: Methods and Protocols, Second Edition aims to be useful to many researchers, inspire them and help them to go single molecule. .
700	1		_aPeterman, Erwin J. G _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493972708
776	0	8	_iPrinted edition: _z9781493972722
776	0	8	_iPrinted edition: _z9781493984374
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7271-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c234208 _d234208