000			03590nam a22003615i 4500
001			234209
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007			cr nn 008mamaa
008			171010s2018 xxu| s |||| 0|eng d
020			_a9781493972685
024	7		_a10.1007/978-1-4939-7268-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDifference Gel Electrophoresis _bMethods and Protocols _cedited by Kay Ohlendieck.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (X, 318 páginas) _b44 ilustraciones, 24 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1664
505	0		_aTwo Dimensional Gel Electrophoresis and 2D-DIGE -- Comparative DIGE Proteomics -- 2D-DIGE and Fluorescence Image Analysis -- DIGE Analysis Software and Protein Identification Approaches -- Native DIGE: Efficient Tool to Elucidate Protein Interactomes -- Comparative Two-dimensional Fluorescence Gel Electrophoresis -- DIGE-based Phosphoproteomic Analysis -- DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-fixed Paraffin-embedded (FFPE) Tissue -- Comparative 3-sample DIGE Analysis of Skeletal Muscles -- DIGE Analysis of ProteoMiner™ Fractionated Serum/Plasma Samples -- DIGE Analysis of Human Tissues -- DIGE Analysis of Animal Tissues -- Rapid 2D DIGE Proteomic Analysis of Mouse Liver -- Proteomic Analysis of Lung Tissue by DIGE -- Comparative Testis Tissue Proteomics using 2-dye versus 3-dye DIGE Analysis -- DIGE Analysis of Fish Tissues -- Protein Digestion for DIGE Analysis -- Subcellular Fractionation for DIGE-based Proteomics -- DIGE Analysis of Immunodepleted Plasma -- Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments using Enzyme Assays -- Enzyme Assay Methods to Validate DIGE Proteomics Data -- Immunoblot Analysis of DIGE-based Proteomics -- Immunofluorescence Microscopy for DIGE-based Proteomics. .
520			_aThis volume provides a comprehensive study of this key method of gel-based proteomics. Chapters inDifference Gel Electrophoresis: Methods and Protocols detail methods on principles of differential protein labeling, two-dimensional gel electrophoresis, techniques on optimized proteomic workflows, advanced mass spectrometry for protein identification, application of basic biological research, and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aOhlendieck, Kay _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493972678
776	0	8	_iPrinted edition: _z9781493972692
776	0	8	_iPrinted edition: _z9781493984367
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7268-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c234209 _d234209