000			03874nam a22003735i 4500
001			234222
003			ES-VaUE
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007			cr nn 008mamaa
008			171017s2018 xxu| s |||| 0|eng d
020			_a9781493973897
024	7		_a10.1007/978-1-4939-7389-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Plant Endoplasmic Reticulum _bMethods and Protocols _cedited by Chris Hawes, Verena Kriechbaumer.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 265 páginas) _b63 ilustraciones, 51 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1691
505	0		_aLabelling the ER for Light and Fluorescence Microscopy -- 3D Electron Microscopy of the ER -- Characterization of Proteins Localised to Plant ER-PM Contact Sites -- Preparation and Imaging of Specialised ER using Super-resolution and TEM Techniques -- Quantitation of ER Structure and Function -- Long-term Imaging of Endoplasmic Reticulum Morphology in Embryos during Seed Germination -- Dancing with the Stars: Using Image Analysis to Study the Choreography of the Endoplasmic Reticulum and Its Partners and of Movement within its Tubules -- Preparation of Highly Enriched ER Membranes using Free-flow Electrophoresis -- ER Microsome Preparation in Arabidopsis thaliana -- ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis -- 2in1 Vectors Improve in Planta BiFC and FRET Analysis -- Metabolons on the Plant ER -- Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions between the ER and Golgi in Plant Cells -- Protein Biosynthesis and Maturation in the ER -- ER Membrane Protein Interactions using the Split-Ubiquitin System (SUS) -- Analysis of Protein Glycosylation in the ER -- The Unfolded Protein Response -- Unfolded Protein Response in Arabidopsis -- Fluorescence Imaging of Autophagy-mediated Er-to-vacuole Trafficking in Plants -- Imaging the ER and Endomembrane System in Cereal Endosperm. .
520			_aThis volume presents a range of different techniques that have been used to characterize the structure and function of the endoplasmic reticulum (ER) in higher plants. Chapters guide readers through application of modern microscopy techniques by fluorescence and electron microscopy, new protocols for analysing ER network structure, methods to purify and analyse ER membrane structure and to study protein glycosylation, protocols to study the unfolded protein response, and the role of the ER in autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, The Plant Endoplasmic Reticulum: Methods and Protocols aims to ensure successful results in the further study of this vital field. .
700	1		_aHawes, Chris _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aKriechbaumer, Verena _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493973880
776	0	8	_iPrinted edition: _z9781493973903
776	0	8	_iPrinted edition: _z9781493984701
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7389-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c234222 _d234222