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020 _a9781493974016
024 7 _a10.1007/978-1-4939-7401-6
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aCancer Stem Cells
_bMethods and Protocols
_cedited by Gianpaolo Papaccio, Vincenzo Desiderio.
250 _a1st edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (XII, 223 páginas)
_b30 ilustraciones, 13 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1692
505 0 _aIntroduction to Cancer Stem Cells: Past, Present, and Future -- Surface Markers for the Identification of Cancer Stem Cells -- The Role of CD44 and Cancer Stem Cells -- Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay -- Isolation of Cancer Stem Cells by Side Population Method -- Self-Renewal and CSCs In Vitro Enrichment: Growth as Floating Spheres -- In Vitro Tumorigenic Assay: The Tumor Spheres Assay -- In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells -- Xenograft as In Vivo Experimental Model -- How to Assess Drug Resistance in Cancer Stem Cells -- Tumor Tissue Analogs for Assessment of Radioresistance in Cancer Stem Cells -- Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML) -- Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System -- Determination of miRNAs from Cancer Stem Cells Using a Low Density Array Platform -- Assessing DNA Methylation in Cancer Stem Cells -- Histones Acetylation and Cancer Stem Cells (CSCs) -- Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods -- Circulating Tumor Cells.
520 _aThis detailed volume gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells. These include surface marker expression, side population, spheres formation, ALDH activity, and numerous others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert tips and troubleshooting advice to avoid potential pitfalls.  Comprehensive and practical, Cancer Stem Cells: Methods and Protocols provides researchers with the techniques they will need in order to pursue vital research into this complex field of study. .
700 1 _aPapaccio, Gianpaolo
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aDesiderio, Vincenzo
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493974009
776 0 8 _iPrinted edition:
_z9781493974023
776 0 8 _iPrinted edition:
_z9781493984749
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7401-6
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2018
999 _c234224
_d234224