000			04183nam a22003855i 4500
001			234232
003			ES-VaUE
005			20221220020549.0
007			cr nn 008mamaa
008			171209s2018 xxu| s |||| 0|eng d
020			_a9781493975143
024	7		_a10.1007/978-1-4939-7514-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNext Generation Sequencing _bMethods and Protocols _cedited by Steven R. Head, Phillip Ordoukhanian, Daniel R. Salomon.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XIII, 266 páginas) _b51 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1712
505	0		_aAn Integrated Polysome Profiling and Ribosome Profiling Method to Investigate in vivo Translatome -- Measuring Nascent Transcripts by Nascent-seq -- Genome-wide Copy Number Alteration Detection in Preimplantation Genetic Diagnosis -- Multiplexed Targeted Sequencing for Oxford Nanopore MinION; A Detailed Library Preparation Procedure -- Hi-Plex for Simple, Accurate, and Cost-effective Amplicon-based Targeted DNA Sequencing -- ClickSeq: Replacing Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Chimeras -- Genome-wide Analysis of DNA Methylation in Single Cells Using a Post-bisulfite Adapter Tagging Approach -- Sequencing of Genomes from Environmental Single Cells -- SNP Discovery from Single and Multiplex Genome Assemblies of Non-model Organisms -- CleanTag Adapters Improve Small RNA Next-Generation Sequencing Library Preparation by Reducing Adapter Dimers -- Sampling, Extraction, and High-Throughput Sequencing Methods for Environmental Microbial and Viral Communities -- A Bloody Primer Analysis of RNA-Seq from Tissue Admixtures -- Next-Generation Sequencing of Genome-wide CRISPR Screens -- Gene Profiling and T Cell Receptor Sequencing from Antigen-specific CD4 T Cells -- Investigate Global Chromosomal Interaction by Hi-C in Human Naïve CD4 T Cells -- Primer Extension, Capture, and On-bead cDNA Ligation: An Efficient RNAseq Library Prep Method for Determining Reverse Transcription Termination Sites. .
520			_aThis volume covers a wide range of various fields of research, with the common thread being Next Generation Sequencing (NGS) related methods and applications, as well as analysis and interpretation of the data obtained. Chapters guide readers through the highly dynamic processes of translational and transcriptional profiling of a cell, method to detect copy number alterations (CNAs), targeted sequencing applications,  method called "Hi-Plex" to characterize known polymorphic loci, single-cell of DNA or RNA, identify and characterize rare circulating CD4 T cells, and computational pipeline for RNAseq analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Next Generation Sequencing: Methods and Protocols aims to be useful and informative for further study into this vital field. .
700	1		_aHead, Steven R _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aOrdoukhanian, Phillip _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSalomon, Daniel R _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493975129
776	0	8	_iPrinted edition: _z9781493975136
776	0	8	_iPrinted edition: _z9781493985074
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7514-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c234232 _d234232