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020 _a9781493976720
024 7 _a10.1007/978-1-4939-7672-0
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlant Senescence
_bMethods and Protocols
_cedited by Yongfeng Guo.
250 _a1st edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (XII, 367 páginas)
_b49 ilustraciones, 32 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1744
505 0 _aConcepts and Types of Senescence in Plants -- Initiation, Progression, and Genetic Manipulation of Leaf Senescence -- Phenotypic Analysis and Molecular Markers of Leaf Senescence -- Investigation of Petal Senescence by TRV-Mediated Virus-Induced Gene Silencing in Rose -- Phenotypic Analysis and Molecular Markers of Plant Nodule Senescence -- Quantitative Analysis of Floral Organ Abscission in Arabidopsis via a Petal Breakstrength Assay -- Characterization of Climacteric and Non-Climacteric Fruit Ripening -- Ethylene Treatment in Studying Leaf Senescence in Arabidopsis -- The Assay of Abscisic Acid-Induced Stomata Movement in Leaf Senescence -- The EPR Method for Detecting Nitric Oxide in Plant Senescence -- Hormone Treatments in Studying Leaf Senescence -- Methods to Study Darkness-Induced Leaf Senescence -- Salt Treatments and Induction of Senescence -- Methods for Elucidation of Plant Senescence in Response to C/N-Nutrient Balance -- Study of Cotton Leaf Senescence Induced by Alternaria alternata Infection -- Study of Hydrogen Peroxide as a Senescence-Inducing Signal -- Identification of Postharvest Senescence Regulators through Map-Based Cloning Using Detached Arabidopsis Inflorescences as a Model Tissue -- Chlorophyll and Chlorophyll Catabolite Analysis by HPLC -- Identification and Functional Analysis of Senescence-Associated Genes in Wheat -- Isolation, Purification, and Detection of MicroRNAs in Plant Senescence -- In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay -- Activities of Vacuolar Cysteine Proteases in Plant Senescence -- Study of Autophagy in Plant Senescence -- Isolation of Chloroplasts for In Organelle Protein Degradation Assay -- Plant Cell Walls: Isolation and Monosaccharide Composition Analysis -- Visualizing Morphological Changes of Abscission Zone Cells in Arabidopsis by Scanning Electron Microscope -- RNA-Seq Analysis of the Transcriptome of Leaf Senescence in Tobacco -- Comprehensive Metabolomics Studies of Plant Developmental Senescence.
520 _aThis detailed volume covers a wide variety of techniques either developed specially for plant senescence studies or optimized for studying senescing plants. After an introduction to the topic, the book continues with sections on phenotypic analysis and molecular markers of plant organ senescence, hormonal control of plant senescence, stress-induced senescence, molecular and cellular processes in plant senescence, as well as systems biology approaches. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Plant Senescence: Methods and Protocols aims to provide a useful hand book of standard protocols for plant molecular biologists working on senescence.
700 1 _aGuo, Yongfeng
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493976706
776 0 8 _iPrinted edition:
_z9781493976713
776 0 8 _iPrinted edition:
_z9781493985401
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7672-0
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2018
999 _c234239
_d234239