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020 _a9781493976805
024 7 _a10.1007/978-1-4939-7680-5
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aCellular Heterogeneity
_bMethods and Protocols
_cedited by Natasha S. Barteneva, Ivan A. Vorobjev.
250 _a1st edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (XVI, 363 páginas)
_b87 ilustraciones, 73 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1745
505 0 _aHeterogeneity of Metazoan Cells and Beyond: To Integrative Analysis of Cellular Populations at Single-Cell Level -- Integrating Analysis of Cellular Heterogeneity in High Content Dose-Response Studies -- Image-Based Tracking of Heterogeneous Single Cell Phenotypes -- High-Dimensional Single Cell Analysis with Mass Cytometry -- Spectral and Imaging Flow Cytometry in Phytoplankton Research -- X-Ray Fluorescence Detected Flow Cytometry -- Multiparametric Analysis of Myeloid Populations by Flow Cytometry -- Quantitation of IRF3 Nuclear Translocation in Heterogeneous Cellular Populations from Cervical Tissue Using Imaging Flow Cytometry -- Methods of Study of Neuron Structural Heterogeneity: Flow Cytometry vs. Laser Interferometry -- Usage of Multiparameter Flow Cytometry to Study Microglia and Macrophage Heterogeneity in the Central Nervous System during Neuroinflammation and Neurodegeneration -- Analysis of Microtubule Dynamics Heterogeneity in Cell Culture -- Heterogeneity of Focal Adhesions and Focal Contacts in Motile Fibroblasts -- Laser Tweezers Raman Micro-Spectroscopy of Single Cells and Biological Particles -- Quantification of the Metabolic Heterogeneity in Mycobacterial Cells Through the Measurement of the NADH/NAD+ Ratio Using a Genetically-Encoded Sensor.- Characterizing Cell Heterogeneity Using PCR Fingerprinting of Surface Multigene Families in Protozoan Parasites.- Assessing Carbon Source-Dependent Phenotypic Variability in Pseudomonas putida -- The Retinal Pigment Epithelial Cell Line (ARPE-19) Displays Mosaic Structural Chromosomal Aberrations -- FACS Isolation of Viable Cells in Different Cell Cycle Stages from Asynchronous Culture for RNA-Sequencing -- Measuring Nanoscale Chromatin Heterogeneity with Partial Wave Spectroscopic Microscopy.
520 _aThis volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity analysis techniques, exploring cytometry, imaging and spectroscopy methods, metabolic and molecular biological methods in tracking, as well as chromatin and cell cycle heterogeneity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Authoritative and accessible, Cellular Heterogeneity: Methods and Protocols encourages readers to explore new ways of studying cells that will help lead to exciting new discoveries.
700 1 _aBarteneva, Natasha S
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aVorobjev, Ivan A
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493976799
776 0 8 _iPrinted edition:
_z9781493976812
776 0 8 _iPrinted edition:
_z9781493985418
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7680-5
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2018
999 _c234243
_d234243