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001 234244
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008 180223s2018 xxu| s |||| 0|eng d
020 _a9781493977628
024 7 _a10.1007/978-1-4939-7762-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aIntravital Imaging of Dynamic Bone and Immune Systems
_bMethods and Protocols
_cedited by Masaru Ishii.
250 _a1st edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (XI, 175 páginas)
_b66 ilustraciones, 56 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1763
505 0 _aBone Imaging: Osteoclast and Osteoblast Dynamics -- Intravital Imaging of Mouse Bone Marrow: Hemodynamics and Vascular Permeability -- Bone Imaging: Platelet Formation Dynamics -- Live Imaging of Interstitial T Cell Migration Using Lymph Node Slices -- Two-Photon Imaging of T-Cell Motility in Lymph Nodes: In Vivo and Ex Vivo Approaches -- Imaging the Lymph Node Stroma -- Intravital Imaging of B Cell Responses in Lymph Nodes -- Live Imaging of the Skin Immune Responses: Visualization of the Contact Hypersensitivity Response -- Imaging of Inflammatory Responses in the Mouse Ear Skin -- In Vivo Imaging of Immune Cells in Peyer's Patches -- Intravital Imaging of T Cells Within the Spinal Cord -- Kidney Imaging: Intravital Microscopy -- Intravital Imaging of Liver Cell Dynamics -- Intravital Imaging of the Heart at the Cellular Level Using Two-Photon Microscopy -- Imaging Window Device for Subcutaneous Implantation Tumor -- New Tools for Imaging of Immune Systems: Visualization of Cell Cycle, Cell Death, and Cell Movement by Using the Mice Lines Expressing Fucci, SCAT3.1, and Kaede and KikGR.
520 _aThis detailed volume presents a number of chapters that will aid researchers in jumping the hurdle preventing the popularization of advanced intravital imaging technology usage, namely the complicated experimental protocols. Leading researchers undertaking imaging studies in the field of bone and immune systems have contributed the chapters that aim to guide readers through these complex methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Intravital Imaging of Dynamic Bone and Immune Systems: Methods and Protocols will serve as an idea guide for general readers in order to establish and perform these experiments by their own. .
700 1 _aIshii, Masaru
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493977611
776 0 8 _iPrinted edition:
_z9781493977635
776 0 8 _iPrinted edition:
_z9781493985449
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7762-8
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2018
999 _c234244
_d234244