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020 _a9781493977017
024 7 _a10.1007/978-1-4939-7701-7
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aCell Migration
_bMethods and Protocols
_cedited by Alexis Gautreau.
250 _a1st edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (XIV, 405 páginas)
_b98 ilustraciones, 88 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1749
505 0 _aRandom Migration Assays of Mammalian Cells and Quantitative Analyses of Single Cell Trajectories -- Directional Collective Migration in Wound Healing Assays -- An In Vitro System to Study the Mesenchymal-to-Amoeboid Transition -- An In Vitro System to Study the Epithelial-Mesenchymal Transition In Vitro -- Detection of Migrasomes -- 3D Endothelial Cell Migration -- Transmigration of Leukocytes across Epithelial Monolayers -- Evaluation of Tumor Cell Invasiveness In Vivo: The Chick Chorioallantoic Membrane Assay -- Analysis of Invasion Dynamics of Matrix-Embedded Cells in a Multi-Sample Format -- Using Systems Microscopy to Understand the Emergence of Cell Migration from Cell Organization -- Neuronal Precursor Migration in Ex Vivo Brain Slice Culture -- In Vitro Models to Analyze the Migration of MGE-Derived Interneurons -- Cell Migration in Tissues: Explant Culture and Live Imaging -- Intravital Imaging of Tumor Cell Motility in the Tumor Microenvironment Context -- Using the Zebrafish Embryo to Dissect the Early Steps of Metastasis Cascade -- Analysis of In Vivo Cell Migration in Mosaic Zebrafish Embryos -- Analysis of Cell Shape and Cell Migration of Drosophila Macrophages In Vivo -- Migration of Q Cells in Caenorhabditis elegans -- Imaging the Molecular Machines that Power Cell Migration -- A Biologist-Friendly Method to Analyze Cross-Correlation between Protrusion Dynamics and Membrane Recruitment of Actin Regulators -- Using Single Protein Tracking to Study Cell Migration -- Optogenetics of Cell Migration -- Electrotaxis: Cell Directional Movement in Electric Fields -- Analysis of Random Migration of Dictyostelium Amoeba in Confined or Unconfined Environments -- Neutrophil Chemotaxis in One Droplet of Blood using Microfluidic Assays -- Leukocyte Migration and Deformation in Collagen Gels and Micro-Fabricated Constrictions -- Microfluidic Devices for Examining the Physical Limits of Migration in Confined Environments -- Controlling Confinement and Topology to Study Collective Cell Behaviors.
520 _aThis volume covers various assays and techniques that have been developed to study and characterize the cell migration in vitro, ex vivo, and in vivo. The chapters in this book present readers with the latest protocols to observe, quantify, and control cell migration. Some of the topics explored in this book are: migration in confined environments, microfluidic devices, optogenetics, chemotaxis, electrotaxis, detection of migrasomes, migration of Q cells in Caenorhabditis elegans, of Drosophila macrophages, optogenetics of cell migration, intravital imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Cell Migration: Methods and Protocols is a valuable resource for anyone interested in learning more about this expanding field.
700 1 _aGautreau, Alexis
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493977000
776 0 8 _iPrinted edition:
_z9781493977024
776 0 8 _iPrinted edition:
_z9781493992614
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7701-7
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2018
999 _c234245
_d234245