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020 _a9781592596713
024 7 _a10.1385/1592596711
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aMAP Kinase Signaling Protocols
_cedited by Rony Seger.
250 _a1st edition 2004
264 1 _aTotowa, NJ
_bHumana Press
_c2004
300 _a1 recurso en línea (XII, 332 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v250
505 0 _aThe ERK Cascade As a Prototype of MAPK Signaling Pathways -- Determination of ERK Activity -- Detection of ERK1/2 Activities Using Affinity Reagents -- Activation of SAPKs/JNKs and p38s In Vitro -- Investigating the Cellular BMK1/ERK5 Signaling Pathway -- Pull-Down Assays for Guanosine 5?-Triphosphate-Bound Ras-Like Guanosine 5?-Triphosphatases -- Regulation of MAPK Cascades by Protein Tyrosine Phosphatases -- Use of Inhibitors in the Study of MAPK Signaling -- Structure of MAPKs -- Subcellular Localization of MAPKs -- Study of MAPK Signaling Using Knockout Mice -- Computer Simulation of MAPK Signal Transduction -- Signaling by Growth Factor Receptors -- Activation of MAPKs by G Protein-Coupled Receptors -- Identification of MAPK Substrates by Expression Screening With Solid-Phase Phosphorylation -- Study of Substrate Specificity of MAPKs Using Oriented Peptide Libraries -- Ras Signaling Pathway for Analysis of Protein-Protein Interactions in Yeast and Mammalian Cells -- Methods in Functional Proteomics -- Practical Methods for Deuterium Exchange/Mass Spectrometry -- Use of Xenopus Oocytes and Early Embryos to Study MAPK Signaling -- MAPK Cascades in the Brain.
520 _aMitogen-activated protein kinase (MAPK) signaling cascades initiate highly significant cellular processes and are involved in a large number of diseases, including cancer and diabetes. In MAP Kinase Signaling Protocols, leading researchers with extensive hands-on experience describe in detail both classic and cutting-edge techniques for the detection and measurement of the MAPKs and other components in these cascades. The protocols include methods for the determination of the subcellular localization of these components, the structural and biophysical analysis of the components, and identification of novel components of known and unknown signaling cascades. Additional methods examine the upstream mechanisms of activation of MAPK cascades by various receptors, the mechanisms involved in the downregulation of the MAPK cascades, and identification of targets of the MAPK cascades. A number of techniques use inhibitors in research on MAPK and transgenic mice for studies of MAPK signaling. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and versatile, MAP Kinase Signaling Protocols offers biochemists, cell biologists, physicians, and biotechnologists all the essential laboratory techniques needed to conduct productive studies of MAPK signaling in health and disease, in measuring the influence of drugs, and in a broad range of experimental systems.
700 1 _aSeger, Rony
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617372957
776 0 8 _iPrinted edition:
_z9780896039988
776 0 8 _iPrinted edition:
_z9781489939777
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592596711
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2004
999 _c234246
_d234246