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008			150516s2015 xxu| s |||| 0|eng d
020			_a9781493926879
024	7		_a10.1007/978-1-4939-2687-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCRISPR _bMethods and Protocols _cedited by Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XIV, 366 páginas) _b63 ilustraciones, 37 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1311
505	0		_aInvestigating CRISPR RNA Biogenesis and Function using RNA-seq -- In vitro Co-reconstitution of Cas Protein Complexes -- Analysis of CRISPR pre-crRNA Cleavage -- Annotation and Classification of CRISPR-Cas Systems -- Computational Detection of CRISPR/crRNA Targets -- High-throughput CRISPR typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium -- Spacer-based Macroarrays for CRISPR Genotyping -- Analysis of crRNA using Liquid Chromatography Electrospray Ionisation Mass Spectrometry (LC ESI MS) -- Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection through CRISPR -- Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification Of Target Interference Motifs in Escherichia coli -- Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes -- Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus -- Procedures for Generating CRISPR Mutants With Novel Spacers Acquired from Viruses Or Plasmids -- Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition -- Using the CRISPR-Cas system to Positively Select Mutants In Genes Essential for its Functionl -- Analysis of Nuclease Activity of Cas1 Proteins Against Complex DNA Substrates -- Characterizing Metal-Dependent Nucleases of CRISPR-Cas prokaryotic Adaptive Immunity Systems -- Cas3 Nuclease-Helicase Activity Assays -- Chemical and Enzymatic Footprint Analyses of R-loop Formation by Cascade-crRNA Complex -- Creation and Analysis of a Virome: Using CRISPR Spacers -- Targeted Mutagenesis in Zebrafish using CRISPR RNA-guided Nucleases -- Precise Genome Editing of Drosophila with CRISPR RNA-guided Cas9 -- Targeted Transcriptional Repression in Bacteria using CRISPR Interference (CRISPRi).
520			_aThis volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.
700	1		_aLundgren, Magnus _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aCharpentier, Emmanuelle _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aFineran, Peter C _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493926862
776	0	8	_iPrinted edition: _z9781493926886
776	0	8	_iPrinted edition: _z9781493954285
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2687-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234253 _d234253