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001			234263
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007			cr nn 008mamaa
008			151006s2016 xxu| s |||| 0|eng d
020			_a9781493929320
024	7		_a10.1007/978-1-4939-2932-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aTALENs _bMethods and Protocols _cedited by Ralf Kühn, Wolfgang Wurst, Benedikt Wefers.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XII, 274 páginas) _b65 ilustraciones, 50 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1338
505	0		_aXanthomonas and the TAL effectors:  Nature's Molecular Biologist -- TAL Effector DNA-binding Principles and Specificity -- The Development of TALE Nucleases for Biotechnology -- Online Tools for TALEN Design -- Assembly of Customized TAL Effectors through Advanced ULtiMATE System -- Engineering Customized TALENs Using the Platinum Gate TALEN Kit -- Design, Assembly, and Characterization of TALE-Based Transcriptional Activators and Repressors -- A New Approach to Dissect Nuclear Organization; TALE-mediated Genome Visualization (TGV) -- TALEN Induced Translocations in Human Cells -- Mutagenesis in Newts; Protocol for Iberian Ribbed Newts -- Targeted Mutagenesis in Bombyx mori Using TALENs -- GeneKnockout by Targeted Mutagenesis in a Hemimetabolous Insect, The TwoSpotted Cricket Gryllus bimaculatus, using TALENs -- Methods for TALEN Evaluation, use, and Mutation Detection in the Mosquito Aedes aegypti -- Methods for TALEN-mediated Genomic Manipulations in Drosophila -- Targeted Mutagenesis in Zebrafish by TALENs -- Mutagenesis in Xenopus and Zebrafish using TALENs -- Genome Editing in Mice using TALE Nucleases -- Genome Editing in Rats using TALE Nucleases -- Designer Nuclease-mediated Generation of Knock out THP1 Cells.
520			_aThis volume provides a comprehensive collection of protocols on new technology across various model organisms.  Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aKühn, Ralf _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWurst, Wolfgang _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWefers, Benedikt _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493929313
776	0	8	_iPrinted edition: _z9781493929337
776	0	8	_iPrinted edition: _z9781493949052
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2932-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c234263 _d234263