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008			150130s2015 xxu| s |||| 0|eng d
020			_a9781493923984
024	7		_a10.1007/978-1-4939-2398-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aGene Essentiality _bMethods and Protocols _cedited by Long Jason Lu.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XI, 248 páginas) _b42 ilustraciones, 15 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1279
505	0		_aMicroarray Transposon Tracking for the Mapping of Conditionally Essential Genes in Campylobacter jejuni -- Identifying Essential Streptococcus sanguinis Genes Using Genome-Wide Deletion Mutation -- Defining Essential Genes and Identifying Virulence Factors of Porphyromonas gingivalis by Massively-Parallel Sequencing of Transposon Libraries (Tn-seq) -- Identification of Essential Genes and Synthetic Lethal Gene Combinations in Escherichia coli K-12 -- Identification of Genes Essential for Leptospirosis -- Identifying Essential Genes in Mycobacterium tuberculosis by Global Phenotypic Profiling -- Essential Genes in the Infection Model of Pseudomonas aeruginosa-PCR-Based Signature-Tagged Mutagenesis -- Genome-Wide Synthetic Genetic Screening by Transposon Mutagenesis in Candida albicans -- An Integrated Machine-Learning Model to Predict Prokaryotic Essential Genes -- A Statistical Framework for Improving Genomic Annotations of Transposon Mutagenesis (TM) Assigned Essential Genes -- A Proposed Essential Gene Discovery Pipeline: A Campylobacter jejuni Case Study -- Computational Prediction of Essential Metabolic Genes Using Constraint-Based Approaches -- Three Computational Tools for Predicting Bacterial Essential Genes -- Gene Essentiality Analysis Based on DEG 10, an Updated Database of Essential Genes -- A Novel Essential Domain Perspective for Exploring Gene Essentiality.
520			_aThis volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Gene Essentiality: Methods and Protocols will aid researchers who wish to further our knowledge in this vital field of study.
700	1		_aLu, Long Jason _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493923991
776	0	8	_iPrinted edition: _z9781493923977
776	0	8	_iPrinted edition: _z9781493948628
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2398-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c234264 _d234264