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001			234265
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007			cr nn 008mamaa
008			100301s1996 xxu| s |||| 0|eng d
020			_a9781592595518
024	7		_a10.1385/0896033449 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPCR Sequencing Protocols _cedited by Ralph Rapley.
250			_a1st edition 1996
264		1	_aTotowa, NJ _bHumana Press _c1996
300			_a1 recurso en línea (XI, 221 páginas) _b24 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v65
505	0		_aPreparation and Analysis of DNA Sequencing Gels -- Purification of PCR Products from Agarose Gels for Direct Sequencing -- Enzymatic Fluorescence and Biotin Labeling of Primers for PCR Sequencing -- Direct Sequencing of Double-Stranded PCR Products with the Sequenase Kit and [?-35 S] dATP -- Direct Sequencing by Thermal Asymmetric PCR -- Rapid Sequencing of cDNA Clones Direct Sequencing Using Sequential Linear/Asymmetric PCR -- Direct Sequencing of PCR Products Using Chemiluminescent Detection -- Direct DNA Sequencing of PCR Products Using Magnetic Beads -- Affinity Capture and Solid-Phase Sequencing of Biotinylated PCR Products -- Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing -- Nonradioactive PCR Sequencing Using Digoxigenin -- Silver Sequencing™ -- Direct Sequencing of PCR Products with DNA-Binding Proteins -- PCR Sequencing with the Aid of Detergents -- Direct Sequencing with Highly Degenerate and lnosine-Containing Primers -- Determination of Unknown Genomic Sequences Without Cloning -- DNA Sequencing by the Chemical Method -- Direct PCR Sequencing with Denaturants (Formamide) -- Efficient PCR Production of Single-Stranded DNA Sequencing Templates -- Preparation and Direct Automated Cycle Sequencing of PCR Products -- Solid-Phase Automated Sequencing of PCR-Amplified Genomic DNA -- Cloning PCR Products for Sequencing in M13 Vectors -- Sequencing PCR Products Cloned into M13 Vectors -- Genomic Amplification with Transcript Sequencing (GAWTS) -- DNA Rescue by the Vectorette Method -- Sequencing of (dA:dT) Cloned Mixed PCR Products from Microbial Populations.
520			_aAdvances in bioscience research usually arise as a result of the continu­ ing refinement of existing technologies. However, there are a number of occa­ sions v^rhere newly developed methodologies have a profound effect on nearly all areas of research. Frequently these are techniques that are elegantly simple in concept and require minimal technical manipulation. Two of these revolu­ tionary techniques are the focus ofPCR Sequencing Protocols. The first such technique is enzymatic chain termination sequencing developed by Sanger and his co-workers in Cambridge and reported in 1977. This essentially brought the possibility of deriving nucleotide sequence information in a very short time scale and has been widely accepted in many laboratories as a routine molecular biological research tool. Furthermore, it has not only led to the sequencing of many genes and gene fragments, but has also allowed the tech­ nical means of sequencing the human genome. The second technique that has found widespread acceptance in basic applied research and many routine applications is the polymerase chain reac­ tion. This technique, first reported in 1985 by MuUis and his colleagues, pro­ vides the means to amplify nucleic acid sequence, which immediately proved invaluable in nearly all fields of biological laboratory research. Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase.
700	1		_aRapley, Ralph _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489940384
776	0	8	_iPrinted edition: _z9781489940377
776	0	8	_iPrinted edition: _z9780896033443
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033449 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1996
999			_c234265 _d234265